Effects of propofol on substance P-induced relaxation in isolated human omental arteries and veins
(2000) In European Journal of Anaesthesiology 17(12). p.720-728- Abstract
- To elucidate if an effect of propofol on endothelium-dependent relaxation could contribute to propofol-induced vasodilation, smooth muscle relaxation of isolated human omental artery and vein segments precontracted by endothelin-1 were measured. Substance P induced a concentration-dependent relaxation (mean +/- SEM) in both artery (63 +/-8.4% of precontraction, n = 9) and vein (60+/-11%, n = 7). The relaxation was enhanced by 10(-6) M propofol (artery, 72+/-9.5%, n = 9; vein, 81+/-12%, n = 7) but not affected by 10(-7), 10(-5) and 10(-4) M propofol. In the presence of Nomega-nitro-L-arginine methyl ester (nitric oxide synthase inhibitor), 10(-6) M propofol still enhanced the substance P-induced relaxation in arteries but not veins, whereas... (More)
- To elucidate if an effect of propofol on endothelium-dependent relaxation could contribute to propofol-induced vasodilation, smooth muscle relaxation of isolated human omental artery and vein segments precontracted by endothelin-1 were measured. Substance P induced a concentration-dependent relaxation (mean +/- SEM) in both artery (63 +/-8.4% of precontraction, n = 9) and vein (60+/-11%, n = 7). The relaxation was enhanced by 10(-6) M propofol (artery, 72+/-9.5%, n = 9; vein, 81+/-12%, n = 7) but not affected by 10(-7), 10(-5) and 10(-4) M propofol. In the presence of Nomega-nitro-L-arginine methyl ester (nitric oxide synthase inhibitor), 10(-6) M propofol still enhanced the substance P-induced relaxation in arteries but not veins, whereas 10(-4) M propofol inhibited the relaxation in both arteries (rightward shift of the concentration-response curve) and veins (28+/-7.5%, n = 8). In the presence of potassium chloride (to prevent hyperpolarization), the enhancement of substance P-induced relaxation by 10(-6) M propofol was abolished in both arteries and veins whereas 10(-5) and 10(-4) M propofol reduced the relaxation in arteries (38+/-13% at 10(-5) M, n = 6; 30+/-11% at 10(-4) M, n = 6) but not in veins. These results demonstrate that propofol, at lower, clinically relevant concentrations, promotes endothelium-dependent relaxation mediated via hyperpolarization in human omental arteries and via both nitric oxide and hyperpolarization in human omental veins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1116907
- author
- Bodelsson, Gunilla LU ; Sandstrom, K ; Wallerstedt, S M ; Hidestal, J ; Tornebrandt, K and Bodelsson, Mikael LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- anaesthetics, intravenous, propofol, tachykinins, substance P, arteries, omental, veins, endothelium, vascular.
- in
- European Journal of Anaesthesiology
- volume
- 17
- issue
- 12
- pages
- 720 - 728
- publisher
- European Society of Anaesthesiology
- external identifiers
-
- pmid:11122309
- scopus:0033635351
- ISSN
- 1365-2346
- DOI
- 10.1046/j.1365-2346.2000.00749.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Infection Medicine (BMC) (013024020), Pediatrics/Urology/Gynecology/Endocrinology (013240400)
- id
- 2859934d-7396-4e0c-8b81-7f3ab4ca7d22 (old id 1116907)
- date added to LUP
- 2016-04-01 12:32:28
- date last changed
- 2022-01-27 06:27:34
@article{2859934d-7396-4e0c-8b81-7f3ab4ca7d22,
abstract = {{To elucidate if an effect of propofol on endothelium-dependent relaxation could contribute to propofol-induced vasodilation, smooth muscle relaxation of isolated human omental artery and vein segments precontracted by endothelin-1 were measured. Substance P induced a concentration-dependent relaxation (mean +/- SEM) in both artery (63 +/-8.4% of precontraction, n = 9) and vein (60+/-11%, n = 7). The relaxation was enhanced by 10(-6) M propofol (artery, 72+/-9.5%, n = 9; vein, 81+/-12%, n = 7) but not affected by 10(-7), 10(-5) and 10(-4) M propofol. In the presence of Nomega-nitro-L-arginine methyl ester (nitric oxide synthase inhibitor), 10(-6) M propofol still enhanced the substance P-induced relaxation in arteries but not veins, whereas 10(-4) M propofol inhibited the relaxation in both arteries (rightward shift of the concentration-response curve) and veins (28+/-7.5%, n = 8). In the presence of potassium chloride (to prevent hyperpolarization), the enhancement of substance P-induced relaxation by 10(-6) M propofol was abolished in both arteries and veins whereas 10(-5) and 10(-4) M propofol reduced the relaxation in arteries (38+/-13% at 10(-5) M, n = 6; 30+/-11% at 10(-4) M, n = 6) but not in veins. These results demonstrate that propofol, at lower, clinically relevant concentrations, promotes endothelium-dependent relaxation mediated via hyperpolarization in human omental arteries and via both nitric oxide and hyperpolarization in human omental veins.}},
author = {{Bodelsson, Gunilla and Sandstrom, K and Wallerstedt, S M and Hidestal, J and Tornebrandt, K and Bodelsson, Mikael}},
issn = {{1365-2346}},
keywords = {{anaesthetics; intravenous; propofol; tachykinins; substance P; arteries; omental; veins; endothelium; vascular.}},
language = {{eng}},
number = {{12}},
pages = {{720--728}},
publisher = {{European Society of Anaesthesiology}},
series = {{European Journal of Anaesthesiology}},
title = {{Effects of propofol on substance P-induced relaxation in isolated human omental arteries and veins}},
url = {{http://dx.doi.org/10.1046/j.1365-2346.2000.00749.x}},
doi = {{10.1046/j.1365-2346.2000.00749.x}},
volume = {{17}},
year = {{2000}},
}