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Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro

Anderson, Emma S; Bjartmar, Carl; Eriksson Linsmeier, Cecilia LU ; Westermark, Gunilla and Hildebrand, Claes (2000) In Neuroscience Letters 284(1-2). p.21-24
Abstract
Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of... (More)
Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell culture, T4-O, Myelination, Development, White matter, Spinal cord, Oligodendrocyte, Chicken
in
Neuroscience Letters
volume
284
issue
1-2
pages
21 - 24
publisher
Elsevier
external identifiers
  • pmid:10771152
  • scopus:0034697054
ISSN
0304-3940
DOI
10.1016/S0304-3940(00)00989-7
language
English
LU publication?
no
id
282f0983-8fc6-442f-90e7-39ac415d5cb6 (old id 1116917)
date added to LUP
2008-07-02 13:15:22
date last changed
2017-01-01 04:47:06
@article{282f0983-8fc6-442f-90e7-39ac415d5cb6,
  abstract     = {Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.},
  author       = {Anderson, Emma S and Bjartmar, Carl and Eriksson Linsmeier, Cecilia and Westermark, Gunilla and Hildebrand, Claes},
  issn         = {0304-3940},
  keyword      = {Cell culture,T4-O,Myelination,Development,White matter,Spinal cord,Oligodendrocyte,Chicken},
  language     = {eng},
  number       = {1-2},
  pages        = {21--24},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro},
  url          = {http://dx.doi.org/10.1016/S0304-3940(00)00989-7},
  volume       = {284},
  year         = {2000},
}