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Limitation of Anatomical Integration between Subretinal Transplants and the Host Retina.

Zhang, Yiqin LU ; Arnér, Karin LU ; Ehinger, Berndt LU and Perez, Maria Thereza LU (2003) In Investigative Ophthalmology & Visual Science 44(1). p.324-331
Abstract
Purpose. In previous studies of subretinal transplantation in rabbits, the host photoreceptor layer seemed to prevent the bridging of neuronal fibers between the graft and the host retina. The current study was undertaken to determine whether the same phenomenon occurs in transplants to the subretinal space of the vascularized retina of rats. Bridging of fibers was examined in transplants to animals of different genetic backgrounds (normal versus dystrophic rats), of different ages, and after different survival times.



Methods. Sprague-Dawley (SD) rat retinal tissue from embryonic day (E)18 was subretinally grafted to adult (60-day-old) normal SD rats, to RCS rats (32 and 73 days old), and to adult (60-day-old) transgenic... (More)
Purpose. In previous studies of subretinal transplantation in rabbits, the host photoreceptor layer seemed to prevent the bridging of neuronal fibers between the graft and the host retina. The current study was undertaken to determine whether the same phenomenon occurs in transplants to the subretinal space of the vascularized retina of rats. Bridging of fibers was examined in transplants to animals of different genetic backgrounds (normal versus dystrophic rats), of different ages, and after different survival times.



Methods. Sprague-Dawley (SD) rat retinal tissue from embryonic day (E)18 was subretinally grafted to adult (60-day-old) normal SD rats, to RCS rats (32 and 73 days old), and to adult (60-day-old) transgenic P23H rats. After various survival times (28–183 days), transplanted retinas were processed for routine histology and immunocytochemistry. Antibodies against calbindin, neuronal nitric oxide synthase (NOS), and protein kinase C (PKC) were used to identify specific retinal cell types and their processes.



Results. The shape and position of the immunoreactive cell bodies indicated that the expected neuronal populations were labeled within the grafts and in the host retina. Labeled neuronal processes were also observed. In each case, NOS-, calbindin-, and PKC-immunolabeled fibers formed bridges between the graft and the host tissues. However, regardless of the extent of host photoreceptor cell loss, the age of the recipient, or the genetic background, bridging fibers were observed only in areas where the host photoreceptor layer was discontinuous or completely missing.



Conclusions. The present study demonstrates that the host photoreceptor layer plays a role in limiting graft–host anatomical integration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Investigative Ophthalmology & Visual Science
volume
44
issue
1
pages
324 - 331
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • wos:000180156300047
  • pmid:12506092
  • scopus:0037247456
ISSN
1552-5783
DOI
10.1167/iovs.02-0132
language
English
LU publication?
yes
id
922b8bc0-5cfa-4f09-896d-524d1e30f00d (old id 111693)
date added to LUP
2007-06-26 13:42:12
date last changed
2018-01-07 09:26:14
@article{922b8bc0-5cfa-4f09-896d-524d1e30f00d,
  abstract     = {Purpose. In previous studies of subretinal transplantation in rabbits, the host photoreceptor layer seemed to prevent the bridging of neuronal fibers between the graft and the host retina. The current study was undertaken to determine whether the same phenomenon occurs in transplants to the subretinal space of the vascularized retina of rats. Bridging of fibers was examined in transplants to animals of different genetic backgrounds (normal versus dystrophic rats), of different ages, and after different survival times.<br/><br>
<br/><br>
Methods. Sprague-Dawley (SD) rat retinal tissue from embryonic day (E)18 was subretinally grafted to adult (60-day-old) normal SD rats, to RCS rats (32 and 73 days old), and to adult (60-day-old) transgenic P23H rats. After various survival times (28–183 days), transplanted retinas were processed for routine histology and immunocytochemistry. Antibodies against calbindin, neuronal nitric oxide synthase (NOS), and protein kinase C (PKC) were used to identify specific retinal cell types and their processes.<br/><br>
<br/><br>
Results. The shape and position of the immunoreactive cell bodies indicated that the expected neuronal populations were labeled within the grafts and in the host retina. Labeled neuronal processes were also observed. In each case, NOS-, calbindin-, and PKC-immunolabeled fibers formed bridges between the graft and the host tissues. However, regardless of the extent of host photoreceptor cell loss, the age of the recipient, or the genetic background, bridging fibers were observed only in areas where the host photoreceptor layer was discontinuous or completely missing.<br/><br>
<br/><br>
Conclusions. The present study demonstrates that the host photoreceptor layer plays a role in limiting graft–host anatomical integration.},
  author       = {Zhang, Yiqin and Arnér, Karin and Ehinger, Berndt and Perez, Maria Thereza},
  issn         = {1552-5783},
  language     = {eng},
  number       = {1},
  pages        = {324--331},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology & Visual Science},
  title        = {Limitation of Anatomical Integration between Subretinal Transplants and the Host Retina.},
  url          = {http://dx.doi.org/10.1167/iovs.02-0132},
  volume       = {44},
  year         = {2003},
}