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Regional specification of neurosphere cultures derived from subregions of the embryonic telencephalon.

Parmar, Malin LU ; Skogh, Charlotta; Björklund, Anders LU and Campbell, Kenneth (2002) In Molecular and Cellular Neuroscience 21(4). p.645-656
Abstract
We have studied the molecular specification of precursor cells in expanded neurosphere cultures derived from distinct subregions of the embryonic mouse telencephalon. These regionally derived cultures exhibited differential responses to the mitogens EGF and bFGF, suggesting that the precursors in these cultures were differentially specified as is the case in situ. To examine this further, cultures from each of the telencephalic subregions were expanded in both EGF and bFGF before differentiation. The neurons produced displayed molecular phenotypes similar to those normally derived from each of these regions in vivo. Moreover, analysis of gene expression in the undifferentiated cultures showed that the regionally derived neurospheres... (More)
We have studied the molecular specification of precursor cells in expanded neurosphere cultures derived from distinct subregions of the embryonic mouse telencephalon. These regionally derived cultures exhibited differential responses to the mitogens EGF and bFGF, suggesting that the precursors in these cultures were differentially specified as is the case in situ. To examine this further, cultures from each of the telencephalic subregions were expanded in both EGF and bFGF before differentiation. The neurons produced displayed molecular phenotypes similar to those normally derived from each of these regions in vivo. Moreover, analysis of gene expression in the undifferentiated cultures showed that the regionally derived neurospheres express many of the same developmental control genes as their in vivo counterparts. Taken together, the present findings suggest that precursor cells in neurosphere cultures, derived from distinct subregions of the embryonic telencephalon, maintain at least certain aspects of their molecular specification, even after significant expansion in vitro. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Neuroscience
volume
21
issue
4
pages
645 - 656
publisher
Elsevier
external identifiers
  • wos:000180026300011
  • pmid:12504597
  • scopus:0036942868
ISSN
1044-7431
DOI
10.1006/mcne.2002.1204
language
English
LU publication?
yes
id
bd452aba-d1d0-4870-82b9-f2316cbcad33 (old id 111730)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12504597&dopt=Abstract
date added to LUP
2007-06-26 12:28:55
date last changed
2017-07-30 03:41:32
@article{bd452aba-d1d0-4870-82b9-f2316cbcad33,
  abstract     = {We have studied the molecular specification of precursor cells in expanded neurosphere cultures derived from distinct subregions of the embryonic mouse telencephalon. These regionally derived cultures exhibited differential responses to the mitogens EGF and bFGF, suggesting that the precursors in these cultures were differentially specified as is the case in situ. To examine this further, cultures from each of the telencephalic subregions were expanded in both EGF and bFGF before differentiation. The neurons produced displayed molecular phenotypes similar to those normally derived from each of these regions in vivo. Moreover, analysis of gene expression in the undifferentiated cultures showed that the regionally derived neurospheres express many of the same developmental control genes as their in vivo counterparts. Taken together, the present findings suggest that precursor cells in neurosphere cultures, derived from distinct subregions of the embryonic telencephalon, maintain at least certain aspects of their molecular specification, even after significant expansion in vitro.},
  author       = {Parmar, Malin and Skogh, Charlotta and Björklund, Anders and Campbell, Kenneth},
  issn         = {1044-7431},
  language     = {eng},
  number       = {4},
  pages        = {645--656},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Regional specification of neurosphere cultures derived from subregions of the embryonic telencephalon.},
  url          = {http://dx.doi.org/10.1006/mcne.2002.1204},
  volume       = {21},
  year         = {2002},
}