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New and simple procedure for the determination of muramic acid in chemically complex environments by gas chromatography-ion trap tandem mass spectrometry

Bal, Karol LU and Larsson, Lennart LU (2000) In Journal of Chromatography. B 738(1). p.57-65
Abstract
A gas chromatographic-ion trap tandem mass spectrometric method was developed for the quantification of muramic acid, a marker of bacterial peptidoglycan, in environmental and clinical specimens. Samples (bacteria, house dust and urine) were heated in methanolic hydrochloric acid overnight and extracted with hexane for removal of hydrophobic compounds. The aqueous phase was evaporated and heated in acetic anhydride and pyridine after which the product, the acetate derivative, was washed with dilute hydrochloric acid and water. The described method is both rapid and simple to apply, and produces a stable derivative. It should become widely used for measuring peptidoglycan in chemically complex environments.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muramic acid
in
Journal of Chromatography. B
volume
738
issue
1
pages
57 - 65
publisher
Elsevier
external identifiers
  • pmid:10778926
  • scopus:0033955501
ISSN
1387-2273
DOI
10.1016/S0378-4347(99)00494-6
language
English
LU publication?
yes
id
242ef090-7a16-4fcf-b27c-80baf4a719c4 (old id 1117431)
date added to LUP
2016-04-01 16:24:09
date last changed
2020-09-09 10:17:50
@article{242ef090-7a16-4fcf-b27c-80baf4a719c4,
  abstract     = {A gas chromatographic-ion trap tandem mass spectrometric method was developed for the quantification of muramic acid, a marker of bacterial peptidoglycan, in environmental and clinical specimens. Samples (bacteria, house dust and urine) were heated in methanolic hydrochloric acid overnight and extracted with hexane for removal of hydrophobic compounds. The aqueous phase was evaporated and heated in acetic anhydride and pyridine after which the product, the acetate derivative, was washed with dilute hydrochloric acid and water. The described method is both rapid and simple to apply, and produces a stable derivative. It should become widely used for measuring peptidoglycan in chemically complex environments.},
  author       = {Bal, Karol and Larsson, Lennart},
  issn         = {1387-2273},
  language     = {eng},
  number       = {1},
  pages        = {57--65},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {New and simple procedure for the determination of muramic acid in chemically complex environments by gas chromatography-ion trap tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1016/S0378-4347(99)00494-6},
  doi          = {10.1016/S0378-4347(99)00494-6},
  volume       = {738},
  year         = {2000},
}