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Comparison between comparative tuberculin and gamma-interferon tests for the diagnosis of bovine tuberculosis in Ethiopia

Ameni, G; Miörner, Håkan LU ; Roger, F and Tibbo, M (2000) In Tropical Animal Health and Production 32(5). p.267-276
Abstract
A study to determine and compare the sensitivities and specificities of the comparative cervical tuberculin (CCT) and gamma-interferon (IFN-gamma) tests for the diagnosis of bovine tuberculosis was conducted on 30 zebu oxen. The results of the tests were compared with the presence of acid-fast bacilli found by bacteriological culturing and histopathological examinations. The sensitivity and specificity of CCT test were found to be 90.9% and 100%, respectively. Those of the commercial IFN-gamma test were determined to be 95.5% and 87.7%, respectively. No significant differences were found between the sensitivities (Yates' corrected chi 2 = 0.32; p = 0.57) or the specificities (Yates' corrected chi 2 = 2.54; p = 0.11) of the two tests.... (More)
A study to determine and compare the sensitivities and specificities of the comparative cervical tuberculin (CCT) and gamma-interferon (IFN-gamma) tests for the diagnosis of bovine tuberculosis was conducted on 30 zebu oxen. The results of the tests were compared with the presence of acid-fast bacilli found by bacteriological culturing and histopathological examinations. The sensitivity and specificity of CCT test were found to be 90.9% and 100%, respectively. Those of the commercial IFN-gamma test were determined to be 95.5% and 87.7%, respectively. No significant differences were found between the sensitivities (Yates' corrected chi 2 = 0.32; p = 0.57) or the specificities (Yates' corrected chi 2 = 2.54; p = 0.11) of the two tests. Furthermore, a positive correlation (r = 0.76) was recorded between the increase in skin thickness following injection of bovine purified protein derivative (PPD) and the optical density in the gamma-interferon assay with bovine PPD. On the other hand, the correlation (r = 0.47) between the change in skin thickness following injection of avian PPD and the optical density in the gamma-interferon assay with avian PPD was relatively weak. On the basis of this preliminary investigation, it was concluded that the choice between the two tests depends on their cost and simplicity and on livestock management and time factors rather than on their respective diagnostic value. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Tropical Animal Health and Production
volume
32
issue
5
pages
267 - 276
publisher
Springer
external identifiers
  • pmid:11059035
  • scopus:0033832667
ISSN
0049-4747
DOI
10.1023/A:1005271421976
language
English
LU publication?
no
id
ef287e41-30d8-4160-8c74-b5a3b5aa97e0 (old id 1117467)
date added to LUP
2008-06-27 12:39:19
date last changed
2017-08-06 04:30:27
@article{ef287e41-30d8-4160-8c74-b5a3b5aa97e0,
  abstract     = {A study to determine and compare the sensitivities and specificities of the comparative cervical tuberculin (CCT) and gamma-interferon (IFN-gamma) tests for the diagnosis of bovine tuberculosis was conducted on 30 zebu oxen. The results of the tests were compared with the presence of acid-fast bacilli found by bacteriological culturing and histopathological examinations. The sensitivity and specificity of CCT test were found to be 90.9% and 100%, respectively. Those of the commercial IFN-gamma test were determined to be 95.5% and 87.7%, respectively. No significant differences were found between the sensitivities (Yates' corrected chi 2 = 0.32; p = 0.57) or the specificities (Yates' corrected chi 2 = 2.54; p = 0.11) of the two tests. Furthermore, a positive correlation (r = 0.76) was recorded between the increase in skin thickness following injection of bovine purified protein derivative (PPD) and the optical density in the gamma-interferon assay with bovine PPD. On the other hand, the correlation (r = 0.47) between the change in skin thickness following injection of avian PPD and the optical density in the gamma-interferon assay with avian PPD was relatively weak. On the basis of this preliminary investigation, it was concluded that the choice between the two tests depends on their cost and simplicity and on livestock management and time factors rather than on their respective diagnostic value.},
  author       = {Ameni, G and Miörner, Håkan and Roger, F and Tibbo, M},
  issn         = {0049-4747},
  language     = {eng},
  number       = {5},
  pages        = {267--276},
  publisher    = {Springer},
  series       = {Tropical Animal Health and Production},
  title        = {Comparison between comparative tuberculin and gamma-interferon tests for the diagnosis of bovine tuberculosis in Ethiopia},
  url          = {http://dx.doi.org/10.1023/A:1005271421976},
  volume       = {32},
  year         = {2000},
}