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EGF infusion stimulates the proliferation and migration of embryonic progenitor cells transplanted in the adult rat striatum

Fricker-Gates, R A; Winkler, Christian LU ; Kirik, Deniz LU ; Rosenblad, C; Carpenter, M K and Björklund, Anders LU (2000) In Experimental Neurology 165(2). p.237-247
Abstract
Immature progenitor cells (generated by in vitro propagation) may provide a useful alternative to primary cells (from dissected embryonic tissue) for transplantation if their migratory and proliferative and differentiation properties can be controlled and directed in vivo. In this study E15 murine EGF-responsive progenitor cells were transplanted to the striatum of adult rats. Simultaneously, these animals received continuous infusion of either epidermal growth factor (EGF) or vehicle, to the lateral ventricle, for 8 days. In animals that received EGF, the transplanted progenitors migrated toward the lateral ventricle and proliferated, as evidenced by bromodeoxyuridine incorporation. Progenitor cells transplanted to rats that received... (More)
Immature progenitor cells (generated by in vitro propagation) may provide a useful alternative to primary cells (from dissected embryonic tissue) for transplantation if their migratory and proliferative and differentiation properties can be controlled and directed in vivo. In this study E15 murine EGF-responsive progenitor cells were transplanted to the striatum of adult rats. Simultaneously, these animals received continuous infusion of either epidermal growth factor (EGF) or vehicle, to the lateral ventricle, for 8 days. In animals that received EGF, the transplanted progenitors migrated toward the lateral ventricle and proliferated, as evidenced by bromodeoxyuridine incorporation. Progenitor cells transplanted to rats that received vehicle infusions showed neither of these responses. In all animals, transplanted progenitors expressed an immature astrocyte or oligodendrocyte phenotype, the majority of cells being astrocytes. We conclude that EGF stimulates the migration and proliferation of murine progenitor cells in vivo, either directly or indirectly, but does not influence their phenotypic differentiation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Neurology
volume
165
issue
2
pages
237 - 247
publisher
Academic Press
external identifiers
  • pmid:10993684
  • scopus:0033803201
ISSN
0014-4886
DOI
10.1006/exnr.2000.7482
language
English
LU publication?
yes
id
4a48ab7e-de20-4dda-81f7-40ce621b1932 (old id 1118210)
date added to LUP
2008-06-18 13:33:53
date last changed
2017-01-01 04:29:46
@article{4a48ab7e-de20-4dda-81f7-40ce621b1932,
  abstract     = {Immature progenitor cells (generated by in vitro propagation) may provide a useful alternative to primary cells (from dissected embryonic tissue) for transplantation if their migratory and proliferative and differentiation properties can be controlled and directed in vivo. In this study E15 murine EGF-responsive progenitor cells were transplanted to the striatum of adult rats. Simultaneously, these animals received continuous infusion of either epidermal growth factor (EGF) or vehicle, to the lateral ventricle, for 8 days. In animals that received EGF, the transplanted progenitors migrated toward the lateral ventricle and proliferated, as evidenced by bromodeoxyuridine incorporation. Progenitor cells transplanted to rats that received vehicle infusions showed neither of these responses. In all animals, transplanted progenitors expressed an immature astrocyte or oligodendrocyte phenotype, the majority of cells being astrocytes. We conclude that EGF stimulates the migration and proliferation of murine progenitor cells in vivo, either directly or indirectly, but does not influence their phenotypic differentiation.},
  author       = {Fricker-Gates, R A and Winkler, Christian and Kirik, Deniz and Rosenblad, C and Carpenter, M K and Björklund, Anders},
  issn         = {0014-4886},
  language     = {eng},
  number       = {2},
  pages        = {237--247},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {EGF infusion stimulates the proliferation and migration of embryonic progenitor cells transplanted in the adult rat striatum},
  url          = {http://dx.doi.org/10.1006/exnr.2000.7482},
  volume       = {165},
  year         = {2000},
}