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Quantitative evaluation of HIV-1 coreceptor use in the GHOST(3) cell assay

Vödrös, Dalma LU ; Tscherning-Casper, Charlotte; Navea, Leonor; Schols, Dominique; De Clercq, Erik and Fenyö, Eva Maria LU (2001) In Virology 291(1). p.1-11
Abstract
The utility of the GHOST(3) cell assay has been evaluated for testing coreceptor use of primary human immunodeficiency virus type 1 (HIV-1) isolates. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, Bonzo, or the orphan receptor BOB. The Indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or simian immunodeficiency virus, Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected 2 or 3 days after infection by simple fluorescence microscopic... (More)
The utility of the GHOST(3) cell assay has been evaluated for testing coreceptor use of primary human immunodeficiency virus type 1 (HIV-1) isolates. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, Bonzo, or the orphan receptor BOB. The Indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or simian immunodeficiency virus, Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected 2 or 3 days after infection by simple fluorescence microscopic observation, This simplicity is the main advantage of the GHOST(3) cell system and makes it particularly suitable for screening of a large number of isolates, In addition, the efficiency of coreceptor use can be accurately quantitated with flow cytometric analysis. Here, we evaluated the coreceptor use of 59 primary HIV-1 isolates of different subtypes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GHOST(3), GFP, coreceptor, quantitative assay, HIV, SIV
in
Virology
volume
291
issue
1
pages
1 - 11
publisher
Elsevier
external identifiers
  • wos:000173221000001
  • scopus:0035814487
ISSN
1096-0341
DOI
10.1006/viro.2001.1163
language
English
LU publication?
yes
id
0774e1f2-ae41-4ea1-99f7-4b5b649b36fd (old id 1118495)
date added to LUP
2008-07-17 10:08:19
date last changed
2018-01-07 09:24:16
@article{0774e1f2-ae41-4ea1-99f7-4b5b649b36fd,
  abstract     = {The utility of the GHOST(3) cell assay has been evaluated for testing coreceptor use of primary human immunodeficiency virus type 1 (HIV-1) isolates. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, Bonzo, or the orphan receptor BOB. The Indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or simian immunodeficiency virus, Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected 2 or 3 days after infection by simple fluorescence microscopic observation, This simplicity is the main advantage of the GHOST(3) cell system and makes it particularly suitable for screening of a large number of isolates, In addition, the efficiency of coreceptor use can be accurately quantitated with flow cytometric analysis. Here, we evaluated the coreceptor use of 59 primary HIV-1 isolates of different subtypes.},
  author       = {Vödrös, Dalma and Tscherning-Casper, Charlotte and Navea, Leonor and Schols, Dominique and De Clercq, Erik and Fenyö, Eva Maria},
  issn         = {1096-0341},
  keyword      = {GHOST(3),GFP,coreceptor,quantitative assay,HIV,SIV},
  language     = {eng},
  number       = {1},
  pages        = {1--11},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Quantitative evaluation of HIV-1 coreceptor use in the GHOST(3) cell assay},
  url          = {http://dx.doi.org/10.1006/viro.2001.1163},
  volume       = {291},
  year         = {2001},
}