Characterisation and differentiation of lactobacilli by lectin typing
(2001) In Journal of Medical Microbiology 50(12). p.1069-1074- Abstract
- Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L.... (More)
- Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L. acidophilus, which remained predominantly auto-agglutinating (eight of nine strains). The system produced stable and reproducible results under standardised culture conditions. Lactobacilli are important bacteria for use as probiotics and this system may supplement current molecular typing techniques and may help in identification of strains that could be useful in this role. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1118646
- author
- Annuk, Heidi ; Hynes, Sean O. ; Hirmo, Siiri ; Mikelsaar, Marika and Wadström, Torkel LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Medical Microbiology
- volume
- 50
- issue
- 12
- pages
- 1069 - 1074
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000172467000009
- scopus:0035193741
- ISSN
- 0022-2615
- language
- English
- LU publication?
- yes
- id
- f0a224fa-493c-4ffa-a838-6eca6e47dfe6 (old id 1118646)
- date added to LUP
- 2016-04-01 17:07:24
- date last changed
- 2022-04-15 17:21:51
@article{f0a224fa-493c-4ffa-a838-6eca6e47dfe6, abstract = {{Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L. acidophilus, which remained predominantly auto-agglutinating (eight of nine strains). The system produced stable and reproducible results under standardised culture conditions. Lactobacilli are important bacteria for use as probiotics and this system may supplement current molecular typing techniques and may help in identification of strains that could be useful in this role.}}, author = {{Annuk, Heidi and Hynes, Sean O. and Hirmo, Siiri and Mikelsaar, Marika and Wadström, Torkel}}, issn = {{0022-2615}}, language = {{eng}}, number = {{12}}, pages = {{1069--1074}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Journal of Medical Microbiology}}, title = {{Characterisation and differentiation of lactobacilli by lectin typing}}, volume = {{50}}, year = {{2001}}, }