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Characterisation and differentiation of lactobacilli by lectin typing

Annuk, Heidi; Hynes, Sean O.; Hirmo, Siiri; Mikelsaar, Marika and Wadström, Torkel LU (2001) In Journal of Medical Microbiology 50(12). p.1069-1074
Abstract
Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L.... (More)
Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L. acidophilus, which remained predominantly auto-agglutinating (eight of nine strains). The system produced stable and reproducible results under standardised culture conditions. Lactobacilli are important bacteria for use as probiotics and this system may supplement current molecular typing techniques and may help in identification of strains that could be useful in this role. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Medical Microbiology
volume
50
issue
12
pages
1069 - 1074
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000172467000009
  • scopus:0035193741
ISSN
0022-2615
language
English
LU publication?
yes
id
f0a224fa-493c-4ffa-a838-6eca6e47dfe6 (old id 1118646)
date added to LUP
2008-06-23 16:38:12
date last changed
2018-01-07 09:46:10
@article{f0a224fa-493c-4ffa-a838-6eca6e47dfe6,
  abstract     = {Lactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L. acidophilus, which remained predominantly auto-agglutinating (eight of nine strains). The system produced stable and reproducible results under standardised culture conditions. Lactobacilli are important bacteria for use as probiotics and this system may supplement current molecular typing techniques and may help in identification of strains that could be useful in this role.},
  author       = {Annuk, Heidi and Hynes, Sean O. and Hirmo, Siiri and Mikelsaar, Marika and Wadström, Torkel},
  issn         = {0022-2615},
  language     = {eng},
  number       = {12},
  pages        = {1069--1074},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Medical Microbiology},
  title        = {Characterisation and differentiation of lactobacilli by lectin typing},
  volume       = {50},
  year         = {2001},
}