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Analytical considerations for free protein S assays in protein S deficiency

Persson, Kristina LU ; Hillarp, Andreas LU and Dahlbäck, Björn LU (2001) In Thrombosis and Haemostasis 86(5). p.1144-1147
Abstract
Protein S is an anticoagulant protein that circulates in plasma in complex with C4b-binding protein; (C4BP) or in free form. Deficiency of protein S increases the risk of venous thrombosis. Measurement of free protein S, as compared to total levels, has been shown to be superior for prediction of protein S deficiency. We studied the effects of different handling protocols for an immuno- and a ligand (C4BP)-based assay for free protein S. When the assay was performed at 37 degrees C, the levels of free protein S in plasma from protein S deficient patients were approximately twice those obtained at room temperature. The reason for this phenomenon was that plasmas from protein S deficient patients exhibited a time-, temperature-, and... (More)
Protein S is an anticoagulant protein that circulates in plasma in complex with C4b-binding protein; (C4BP) or in free form. Deficiency of protein S increases the risk of venous thrombosis. Measurement of free protein S, as compared to total levels, has been shown to be superior for prediction of protein S deficiency. We studied the effects of different handling protocols for an immuno- and a ligand (C4BP)-based assay for free protein S. When the assay was performed at 37 degrees C, the levels of free protein S in plasma from protein S deficient patients were approximately twice those obtained at room temperature. The reason for this phenomenon was that plasmas from protein S deficient patients exhibited a time-, temperature-, and dilution-dependent increase in free protein S, which, was more pronounced than corresponding dilution of the normal plasma that was used to create the standard curve. These findings demonstrate the importance of assay procedure and sample handling in assays for free protein S. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Thrombosis and Haemostasis
volume
86
issue
5
pages
1144 - 1147
publisher
F K Schattauer Verlag Gmbh
external identifiers
  • wos:000172272000004
  • scopus:0035167456
ISSN
0340-6245
language
English
LU publication?
yes
id
bece6c23-f9f8-4baa-aec0-e027d331aca7 (old id 1118664)
date added to LUP
2008-07-09 11:09:40
date last changed
2018-05-29 10:18:08
@article{bece6c23-f9f8-4baa-aec0-e027d331aca7,
  abstract     = {Protein S is an anticoagulant protein that circulates in plasma in complex with C4b-binding protein; (C4BP) or in free form. Deficiency of protein S increases the risk of venous thrombosis. Measurement of free protein S, as compared to total levels, has been shown to be superior for prediction of protein S deficiency. We studied the effects of different handling protocols for an immuno- and a ligand (C4BP)-based assay for free protein S. When the assay was performed at 37 degrees C, the levels of free protein S in plasma from protein S deficient patients were approximately twice those obtained at room temperature. The reason for this phenomenon was that plasmas from protein S deficient patients exhibited a time-, temperature-, and dilution-dependent increase in free protein S, which, was more pronounced than corresponding dilution of the normal plasma that was used to create the standard curve. These findings demonstrate the importance of assay procedure and sample handling in assays for free protein S.},
  author       = {Persson, Kristina and Hillarp, Andreas and Dahlbäck, Björn},
  issn         = {0340-6245},
  language     = {eng},
  number       = {5},
  pages        = {1144--1147},
  publisher    = {F K Schattauer Verlag Gmbh},
  series       = {Thrombosis and Haemostasis},
  title        = {Analytical considerations for free protein S assays in protein S deficiency},
  volume       = {86},
  year         = {2001},
}