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The role of glycosaminoglycan binding of staphylococci in attachment to eukaryotic host cells

Fallgren, Corina; Andersson, Annika and Ljungh, Åsa LU (2001) In Current Microbiology 43(1). p.57-63
Abstract
Attachment of microorganisms to host cells is believed to be a critical early step in microbial pathogenesis. The aim of the study was to determine the role of the known glycosaminoglycan (GAG) binding activity of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in their attachment to six different eukaryotic cell lines. Three staphylococcal species expressing GAG binding capacity-S. aureus, S. epidermidis, and S. hemolyticus-were chosen for investigation. Six different eukaryotic cell lines, endothelial HUVEC and EA. hy 926 cells, epithelial A549 and HeLa S3 cells, fibroblasts HEL Sp 12 and macrophages J774.A1, were included. A modified ELISA with biotinylated bacteria was used for estimating the adhesion of staphylococci... (More)
Attachment of microorganisms to host cells is believed to be a critical early step in microbial pathogenesis. The aim of the study was to determine the role of the known glycosaminoglycan (GAG) binding activity of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in their attachment to six different eukaryotic cell lines. Three staphylococcal species expressing GAG binding capacity-S. aureus, S. epidermidis, and S. hemolyticus-were chosen for investigation. Six different eukaryotic cell lines, endothelial HUVEC and EA. hy 926 cells, epithelial A549 and HeLa S3 cells, fibroblasts HEL Sp 12 and macrophages J774.A1, were included. A modified ELISA with biotinylated bacteria was used for estimating the adhesion of staphylococci to each of the cell lines. Our results showed that staphylococci adhered to each of the cell lines studied, although the binding of CoNS strains to epithelial cells was lower than to the other cells. The attachment to all cell types could be partially decreased by pretreatment of the bacteria with various polysulfated agents (highest inhibition was 60%), as well as by chlorate and heparitinase treatment of the cells. These observations may suggest that at least one mode of staphylococcal attachment utilizes GAG chains present on the surface of virtually all adherent cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Microbiology
volume
43
issue
1
pages
57 - 63
publisher
Springer
external identifiers
  • wos:000169175800011
  • scopus:0034971450
ISSN
0343-8651
DOI
10.1007/s002840010260
language
English
LU publication?
yes
id
4eb664a5-a163-421d-bd88-0fccb1019d37 (old id 1119169)
date added to LUP
2008-06-27 10:37:51
date last changed
2018-05-29 12:17:08
@article{4eb664a5-a163-421d-bd88-0fccb1019d37,
  abstract     = {Attachment of microorganisms to host cells is believed to be a critical early step in microbial pathogenesis. The aim of the study was to determine the role of the known glycosaminoglycan (GAG) binding activity of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in their attachment to six different eukaryotic cell lines. Three staphylococcal species expressing GAG binding capacity-S. aureus, S. epidermidis, and S. hemolyticus-were chosen for investigation. Six different eukaryotic cell lines, endothelial HUVEC and EA. hy 926 cells, epithelial A549 and HeLa S3 cells, fibroblasts HEL Sp 12 and macrophages J774.A1, were included. A modified ELISA with biotinylated bacteria was used for estimating the adhesion of staphylococci to each of the cell lines. Our results showed that staphylococci adhered to each of the cell lines studied, although the binding of CoNS strains to epithelial cells was lower than to the other cells. The attachment to all cell types could be partially decreased by pretreatment of the bacteria with various polysulfated agents (highest inhibition was 60%), as well as by chlorate and heparitinase treatment of the cells. These observations may suggest that at least one mode of staphylococcal attachment utilizes GAG chains present on the surface of virtually all adherent cells.},
  author       = {Fallgren, Corina and Andersson, Annika and Ljungh, Åsa},
  issn         = {0343-8651},
  language     = {eng},
  number       = {1},
  pages        = {57--63},
  publisher    = {Springer},
  series       = {Current Microbiology},
  title        = {The role of glycosaminoglycan binding of staphylococci in attachment to eukaryotic host cells},
  url          = {http://dx.doi.org/10.1007/s002840010260},
  volume       = {43},
  year         = {2001},
}