Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets
(2001) In Biochemical and Biophysical Research Communications 283(2). p.454-459- Abstract
- gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma... (More)
- gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma -carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin, The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1119255
- author
- Stenberg, Leisa LU ; Nilsson, Elise LU ; Ljungberg, Otto LU ; Stenflo, Johan LU and Brown, Mark A. LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- γ-carboxylation, Gla, pancreas, protein S, prothrombin, vitamin K
- in
- Biochemical and Biophysical Research Communications
- volume
- 283
- issue
- 2
- pages
- 454 - 459
- publisher
- Elsevier
- external identifiers
-
- wos:000168612700032
- scopus:0034809495
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.2001.4808
- language
- English
- LU publication?
- yes
- id
- 5a758a94-3f29-4c2e-be2a-238f0ce09b15 (old id 1119255)
- date added to LUP
- 2016-04-01 17:08:29
- date last changed
- 2022-01-29 00:39:13
@article{5a758a94-3f29-4c2e-be2a-238f0ce09b15, abstract = {{gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma -carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin, The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins.}}, author = {{Stenberg, Leisa and Nilsson, Elise and Ljungberg, Otto and Stenflo, Johan and Brown, Mark A.}}, issn = {{1090-2104}}, keywords = {{γ-carboxylation; Gla; pancreas; protein S; prothrombin; vitamin K}}, language = {{eng}}, number = {{2}}, pages = {{454--459}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets}}, url = {{http://dx.doi.org/10.1006/bbrc.2001.4808}}, doi = {{10.1006/bbrc.2001.4808}}, volume = {{283}}, year = {{2001}}, }