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Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets

Stenberg, Leisa LU ; Nilsson, Elise LU ; Ljungberg, Otto LU ; Stenflo, Johan LU and Brown, Mark A. LU (2001) In Biochemical and Biophysical Research Communications 283(2). p.454-459
Abstract
gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma... (More)
gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma -carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin, The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
γ-carboxylation, Gla, pancreas, protein S, prothrombin, vitamin K
in
Biochemical and Biophysical Research Communications
volume
283
issue
2
pages
454 - 459
publisher
Elsevier
external identifiers
  • wos:000168612700032
  • scopus:0034809495
ISSN
1090-2104
DOI
10.1006/bbrc.2001.4808
language
English
LU publication?
yes
id
5a758a94-3f29-4c2e-be2a-238f0ce09b15 (old id 1119255)
date added to LUP
2008-07-15 10:21:58
date last changed
2018-06-24 04:44:29
@article{5a758a94-3f29-4c2e-be2a-238f0ce09b15,
  abstract     = {gamma -Carboxylated proteins were detected in normal human pancreas by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues, Staining appeared to be localized to the glucagon-secreting alpha -cells in the islets of Langerhans, Consistent with this, sections from a glucagonoma were stained much more intensely with the M3B antibody than those from an insulinoma. A murine alpha -cell line (alpha TC1 Clone 9) was cultured and gamma -carboxylated polypeptides, identified immunologically as prothrombin, protein S and (tentatively) Gas6, were isolated from the intracellular compartment by chromatography on an M3B-coupled resin, As in liver, prothrombin is synthesized by alpha -cells as a gamma -carboxylated zymogen that can be cleaved by ecarin to form an active serine protease that is inhibited by hirudin, The pancreas thus appears to be a novel site of synthesis for certain vitamin K-dependent proteins.},
  author       = {Stenberg, Leisa and Nilsson, Elise and Ljungberg, Otto and Stenflo, Johan and Brown, Mark A.},
  issn         = {1090-2104},
  keyword      = {γ-carboxylation,Gla,pancreas,protein S,prothrombin,vitamin K},
  language     = {eng},
  number       = {2},
  pages        = {454--459},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Synthesis of gamma-carboxylated polypeptides by alpha-cells of the pancreatic islets},
  url          = {http://dx.doi.org/10.1006/bbrc.2001.4808},
  volume       = {283},
  year         = {2001},
}