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Characterization of Lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern Sweden

Ornstein, Katharina LU ; Berglund, Johan LU ; Nilsson, Ingrid; Norrby, Ragnar and Bergström, Sven (2001) In Journal of Clinical Microbiology 39(4). p.1294-1298
Abstract
Southern Sweden is an area of Lyme borreliosis (LB) endemicity, with an incidence of 9 cases per 100,000 inhabitants. The most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). There was no record of human Borrelia strains being isolated front patients in this region before the prospective study reported here. Borrelia spirochetes were isolated from skin and cerebrospinal fluid (CSF) from LB patients living in the region. A total of 39 strains were characterized by OspA serotype analysis, species-specific PCR, and signature nucleotide analysis of the 16S rRNA gene. Of 33 skin isolates, 31 (93.9%) were Borrelia afzelii strains and 2 (6.1%) were Borrelia garinii strains. Of six CSF isolates, five (83,3%)... (More)
Southern Sweden is an area of Lyme borreliosis (LB) endemicity, with an incidence of 9 cases per 100,000 inhabitants. The most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). There was no record of human Borrelia strains being isolated front patients in this region before the prospective study reported here. Borrelia spirochetes were isolated from skin and cerebrospinal fluid (CSF) from LB patients living in the region. A total of 39 strains were characterized by OspA serotype analysis, species-specific PCR, and signature nucleotide analysis of the 16S rRNA gene. Of 33 skin isolates, 31 (93.9%) were Borrelia afzelii strains and 2 (6.1%) were Borrelia garinii strains. Of six CSF isolates, five (83,3%) were B. garinii and one (16.7%) was B. afzelii, Neither Borrelia burgdorferi sensu stricto strains nor multiple infections were observed, The B. afzelii isolates were of OspA serotype 2, Three B, garinii strains were of OspA serotype 5, and the remaining four strains were of OspA serotype 6, All of the B, garinii strains belonged to the same 16S ribosomal DNA ribotype class, Our findings agree with earlier findings front other geographic regions in Europe where B. afzelii and B, garinii have been recovered predominately from skin and CSF cultures, respectively, To further study the possible presence in Sweden of the genotype B. burgdorferi sensu stricto, which is known to be present in Europe and to occur predominately in patients with Lyme arthritis, molecular detection of Borrelia-specific DNA in synovial samples from LS me arthritis patients should he performed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
39
issue
4
pages
1294 - 1298
publisher
American Society for Microbiology
external identifiers
  • wos:000167946500016
  • scopus:0035056295
ISSN
1098-660X
DOI
10.1128/JCM.39.4.1294-1298.2001
language
English
LU publication?
yes
id
4b7a854b-471a-4082-882a-b74734fed4eb (old id 1119322)
date added to LUP
2008-07-09 09:41:48
date last changed
2018-05-29 09:47:09
@article{4b7a854b-471a-4082-882a-b74734fed4eb,
  abstract     = {Southern Sweden is an area of Lyme borreliosis (LB) endemicity, with an incidence of 9 cases per 100,000 inhabitants. The most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). There was no record of human Borrelia strains being isolated front patients in this region before the prospective study reported here. Borrelia spirochetes were isolated from skin and cerebrospinal fluid (CSF) from LB patients living in the region. A total of 39 strains were characterized by OspA serotype analysis, species-specific PCR, and signature nucleotide analysis of the 16S rRNA gene. Of 33 skin isolates, 31 (93.9%) were Borrelia afzelii strains and 2 (6.1%) were Borrelia garinii strains. Of six CSF isolates, five (83,3%) were B. garinii and one (16.7%) was B. afzelii, Neither Borrelia burgdorferi sensu stricto strains nor multiple infections were observed, The B. afzelii isolates were of OspA serotype 2, Three B, garinii strains were of OspA serotype 5, and the remaining four strains were of OspA serotype 6, All of the B, garinii strains belonged to the same 16S ribosomal DNA ribotype class, Our findings agree with earlier findings front other geographic regions in Europe where B. afzelii and B, garinii have been recovered predominately from skin and CSF cultures, respectively, To further study the possible presence in Sweden of the genotype B. burgdorferi sensu stricto, which is known to be present in Europe and to occur predominately in patients with Lyme arthritis, molecular detection of Borrelia-specific DNA in synovial samples from LS me arthritis patients should he performed.},
  author       = {Ornstein, Katharina and Berglund, Johan and Nilsson, Ingrid and Norrby, Ragnar and Bergström, Sven},
  issn         = {1098-660X},
  language     = {eng},
  number       = {4},
  pages        = {1294--1298},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Characterization of Lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern Sweden},
  url          = {http://dx.doi.org/10.1128/JCM.39.4.1294-1298.2001},
  volume       = {39},
  year         = {2001},
}