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Beneficial effects of low-dose prostacyclin on cat intestinal perfusion during endotoxemia as evaluated with microdialysis and oxygen transport variables

Möller, Alma LU and Grände, Per-Olof LU (2001) In Critical Care Medicine 29(2). p.351-358
Abstract
Objective: To evaluate the effects of low-dose prostacyclin on intestinal perfusion during endotoxemia. Design: A randomized, blinded experimental study. Setting: A university laboratory. Subjects: Sixteen anesthetized cats. Interventions: The animals received endotoxin by continuous intravenous infusion (0.5 mg/kg plus 0.5 mg kg(-1).hr(-1)) and a continuous volume replacement throughout the experiment. Four hours after the start of endotoxin, the animals were randomized to receive an infusion of either prostacyclin at a dose of 1 ng.kg(-1).min(-1) (prostacyclin group) or vehicle (control group) during the next 4 hrs. Measurements and Main Results: Intestinal vascular resistance was calculated from systemic arterial pressure, central... (More)
Objective: To evaluate the effects of low-dose prostacyclin on intestinal perfusion during endotoxemia. Design: A randomized, blinded experimental study. Setting: A university laboratory. Subjects: Sixteen anesthetized cats. Interventions: The animals received endotoxin by continuous intravenous infusion (0.5 mg/kg plus 0.5 mg kg(-1).hr(-1)) and a continuous volume replacement throughout the experiment. Four hours after the start of endotoxin, the animals were randomized to receive an infusion of either prostacyclin at a dose of 1 ng.kg(-1).min(-1) (prostacyclin group) or vehicle (control group) during the next 4 hrs. Measurements and Main Results: Intestinal vascular resistance was calculated from systemic arterial pressure, central venous pressure, and superior mesenteric artery blood flow, and intestinal oxygen delivery and uptake were calculated from superior mesenteric artery and vein blood samples and blood flow. Interstitial lactate, pyruvate, glucose, and glycerol in the ileal wall were measured by using microdialysis. There were no differences in baseline values between the groups, Systemic blood pressure decreased initially but recovered and remained stable in both groups. In the control group, intestinal vascular resistance increased from 10.9 +/- 1.0 to 24.7 +/- 5.3 mm Hg.mL.min(-1).kg(-1) (p < .05) at 8 hrs, and oxygen delivery decreased from 2.6 +/- 0.2 to 1.3 +/- 0.3 ml min(-1).kg(-1) (p <.05), Simultaneously, microdialysis lactate increased from 1.6 +/- 0.1 to 3.6 +/- 0.5 mmol/L (p <.05) with concomitant pyruvate increase and unchanged lactate/pyruvate ratio. Blood lactate increased and ph decreased. In the prostacyclin group at 8 hrs, intestinal vascular resistance of 6.9 +/- 0.8 mm Hg.mL.min(-1) kg(-1) was lower and intestinal oxygen delivery of 3.2 +/- 0.3 was higher (p <.05) than in the control group at 8 hrs. Intestinal oxygen uptake of 0.54 +/- 0.10 mL.min(-1).kg(-1) was higher than in the control group, in which oxygen uptake was 0.26 +/- 0.04 mL.min(-1) kg(-1). Lactate, pyruvate, and pH were normalized at 8 hrs in the prostacyclin group. Conclusion: Low-dose prostacyclin has beneficial effects on small intestinal perfusion during endotoxemia in this experimental cat model. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Critical Care Medicine
volume
29
issue
2
pages
351 - 358
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000167179400020
  • scopus:0035110016
ISSN
1530-0293
language
English
LU publication?
yes
id
1a1c564d-9d2d-4548-bf19-92cab6c4190e (old id 1119537)
date added to LUP
2008-07-08 08:53:08
date last changed
2018-01-07 08:39:28
@article{1a1c564d-9d2d-4548-bf19-92cab6c4190e,
  abstract     = {Objective: To evaluate the effects of low-dose prostacyclin on intestinal perfusion during endotoxemia. Design: A randomized, blinded experimental study. Setting: A university laboratory. Subjects: Sixteen anesthetized cats. Interventions: The animals received endotoxin by continuous intravenous infusion (0.5 mg/kg plus 0.5 mg kg(-1).hr(-1)) and a continuous volume replacement throughout the experiment. Four hours after the start of endotoxin, the animals were randomized to receive an infusion of either prostacyclin at a dose of 1 ng.kg(-1).min(-1) (prostacyclin group) or vehicle (control group) during the next 4 hrs. Measurements and Main Results: Intestinal vascular resistance was calculated from systemic arterial pressure, central venous pressure, and superior mesenteric artery blood flow, and intestinal oxygen delivery and uptake were calculated from superior mesenteric artery and vein blood samples and blood flow. Interstitial lactate, pyruvate, glucose, and glycerol in the ileal wall were measured by using microdialysis. There were no differences in baseline values between the groups, Systemic blood pressure decreased initially but recovered and remained stable in both groups. In the control group, intestinal vascular resistance increased from 10.9 +/- 1.0 to 24.7 +/- 5.3 mm Hg.mL.min(-1).kg(-1) (p &lt; .05) at 8 hrs, and oxygen delivery decreased from 2.6 +/- 0.2 to 1.3 +/- 0.3 ml min(-1).kg(-1) (p &lt;.05), Simultaneously, microdialysis lactate increased from 1.6 +/- 0.1 to 3.6 +/- 0.5 mmol/L (p &lt;.05) with concomitant pyruvate increase and unchanged lactate/pyruvate ratio. Blood lactate increased and ph decreased. In the prostacyclin group at 8 hrs, intestinal vascular resistance of 6.9 +/- 0.8 mm Hg.mL.min(-1) kg(-1) was lower and intestinal oxygen delivery of 3.2 +/- 0.3 was higher (p &lt;.05) than in the control group at 8 hrs. Intestinal oxygen uptake of 0.54 +/- 0.10 mL.min(-1).kg(-1) was higher than in the control group, in which oxygen uptake was 0.26 +/- 0.04 mL.min(-1) kg(-1). Lactate, pyruvate, and pH were normalized at 8 hrs in the prostacyclin group. Conclusion: Low-dose prostacyclin has beneficial effects on small intestinal perfusion during endotoxemia in this experimental cat model.},
  author       = {Möller, Alma and Grände, Per-Olof},
  issn         = {1530-0293},
  language     = {eng},
  number       = {2},
  pages        = {351--358},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Critical Care Medicine},
  title        = {Beneficial effects of low-dose prostacyclin on cat intestinal perfusion during endotoxemia as evaluated with microdialysis and oxygen transport variables},
  volume       = {29},
  year         = {2001},
}