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A functional prothrombin gene product is synthesized by human kidney cells

Stenberg, Leisa LU ; Brown, Mark A. LU ; Nilsson, Elise LU ; Ljungberg, Otto LU and Stenflo, Johan LU (2001) In Biochemical and Biophysical Research Communications 280(4). p.1036-1041
Abstract
gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by... (More)
gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
kidney, prothrombin, γ-carboxyglutamate, HEK293 cells, growth arrest-specific protein 6, nephrolithiasis
in
Biochemical and Biophysical Research Communications
volume
280
issue
4
pages
1036 - 1041
publisher
Elsevier
external identifiers
  • wos:000166941900014
  • scopus:0034815154
ISSN
1090-2104
DOI
10.1006/bbrc.2000.4145
language
English
LU publication?
yes
id
def2b915-5e73-4c64-9f93-ccf0bcd5f80f (old id 1119602)
date added to LUP
2008-07-15 10:17:23
date last changed
2018-05-29 11:40:29
@article{def2b915-5e73-4c64-9f93-ccf0bcd5f80f,
  abstract     = {gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells.},
  author       = {Stenberg, Leisa and Brown, Mark A. and Nilsson, Elise and Ljungberg, Otto and Stenflo, Johan},
  issn         = {1090-2104},
  keyword      = {kidney,prothrombin,γ-carboxyglutamate,HEK293 cells,growth arrest-specific protein 6,nephrolithiasis},
  language     = {eng},
  number       = {4},
  pages        = {1036--1041},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {A functional prothrombin gene product is synthesized by human kidney cells},
  url          = {http://dx.doi.org/10.1006/bbrc.2000.4145},
  volume       = {280},
  year         = {2001},
}