A functional prothrombin gene product is synthesized by human kidney cells
(2001) In Biochemical and Biophysical Research Communications 280(4). p.1036-1041- Abstract
- gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by... (More)
- gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1119602
- author
- Stenberg, Leisa LU ; Brown, Mark A. LU ; Nilsson, Elise LU ; Ljungberg, Otto LU and Stenflo, Johan LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- kidney, prothrombin, γ-carboxyglutamate, HEK293 cells, growth arrest-specific protein 6, nephrolithiasis
- in
- Biochemical and Biophysical Research Communications
- volume
- 280
- issue
- 4
- pages
- 1036 - 1041
- publisher
- Elsevier
- external identifiers
-
- wos:000166941900014
- scopus:0034815154
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.2000.4145
- language
- English
- LU publication?
- yes
- id
- def2b915-5e73-4c64-9f93-ccf0bcd5f80f (old id 1119602)
- date added to LUP
- 2016-04-01 17:10:52
- date last changed
- 2022-01-29 00:55:29
@article{def2b915-5e73-4c64-9f93-ccf0bcd5f80f,
abstract = {{gamma -carboxylated polypeptides were detected in the human kidney by immunohistochemistry with a monoclonal antibody (M3B) specific for gamma -carboxyglutamyl residues. An similar to 70-kDa gamma -carboxylated protein, subsequently identified as prothrombin, was isolated from the intracellular compartment of cultured human embryonic kidney (HEK293) cells by immunoaffinity chromatography on M3B-coupled resin. Immunohistochemical analyses demonstrated that prothrombin and another vitamin K-dependent protein, the growth arrest-specific protein 6, were detectable in human kidney. As in the liver, the kidney synthesizes prothrombin as a zymogen that can be cleaved by ecarin to an amidolytically active serine protease that is inhibited by hirudin, This demonstrates for the first time the de novo synthesis of a full-length, gamma -carboxylated, and functional prothrombin gene product by human kidney cells.}},
author = {{Stenberg, Leisa and Brown, Mark A. and Nilsson, Elise and Ljungberg, Otto and Stenflo, Johan}},
issn = {{1090-2104}},
keywords = {{kidney; prothrombin; γ-carboxyglutamate; HEK293 cells; growth arrest-specific protein 6; nephrolithiasis}},
language = {{eng}},
number = {{4}},
pages = {{1036--1041}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{A functional prothrombin gene product is synthesized by human kidney cells}},
url = {{http://dx.doi.org/10.1006/bbrc.2000.4145}},
doi = {{10.1006/bbrc.2000.4145}},
volume = {{280}},
year = {{2001}},
}