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Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens

Apfalter, Petra; Blasi, Francesco; Boman, Jens; Gaydos, Charlotte A.; Kundi, Michael; Maass, Matthias; Makristathis, Athanasios; Meijer, Adam; Nadrchal, Reinhard and Persson, Kenneth LU , et al. (2001) In Journal of Clinical Microbiology 39(2). p.519-524
Abstract
The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery... (More)
The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used. (Less)
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Journal of Clinical Microbiology
volume
39
issue
2
pages
519 - 524
publisher
American Society for Microbiology
external identifiers
  • wos:000166776100016
  • scopus:0035129962
ISSN
1098-660X
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language
English
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yes
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a75ba3d4-0961-486a-9c2e-fe1d3680976f (old id 1119648)
date added to LUP
2008-06-24 10:53:48
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2018-05-29 09:20:58
@article{a75ba3d4-0961-486a-9c2e-fe1d3680976f,
  abstract     = {The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls were correctly identified to levels of 1, 0.1, and 0.01 inclusion bodies of C. pneumoniae/ml of tissue homogenate by 16 (100%), 11 (69%), and 3 (19%) of the test methods, respectively. Three out of 16 negative controls (19%) were rated positive. Positivity rates for atheroma samples varied between 0 and 60% for the different test methods, with the maximum concordant result for positivity being only 25% for one carotid artery sample. There was no consistent pattern of positive results among the various laboratories, and there was no correlation between the detection rates and the sensitivity of the assay used.},
  author       = {Apfalter, Petra and Blasi, Francesco and Boman, Jens and Gaydos, Charlotte A. and Kundi, Michael and Maass, Matthias and Makristathis, Athanasios and Meijer, Adam and Nadrchal, Reinhard and Persson, Kenneth and Rotter, Manfred L. and Tong, C. Y. William and Stanek, Gerold and Hirschl, Alexander M.},
  issn         = {1098-660X},
  language     = {eng},
  number       = {2},
  pages        = {519--524},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens},
  url          = {http://dx.doi.org/},
  volume       = {39},
  year         = {2001},
}