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Cell-specific processing of chromogranin A in endocrine cells of the rat stomach

Norlén, Per LU ; Curry, William J. ; Björkqvist, Maria LU ; Maule, Aron ; Cunningham, Rodat T. ; Hogg, Robert B. ; Harriott, Pat ; Johnston, Colin F. ; Hutton, John C. and Håkanson, Rolf LU (2001) In Journal of Histochemistry and Cytochemistry 49(1). p.41535-41535
Abstract
The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine Ib) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylsae (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and... (More)
The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine Ib) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylsae (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and CgA-derived peptides in the various endocrine cell populations of the rat stomach. In the oxyntic mucosa, antisera raised against CgA(291-319) and CGA(316-321) immunostained D cells exclusively, whereas antisera raised against bCgA(82-91) and CgA(121-128) immunostained A-like cells and D cells. Antisera raised against CgA(318-349) and CgA(437-448) immunostained ECL cells and A-like cells, but not D cells. In the antrum, antisera against CgA(291-319) immunostained D cells, and antisera against CgA(351-356) immunostained G cells. Our observations suggest that each individual endocrine cell type in the rat stomach generates a unique mixture of CgA-derived peptides, probably reflecting cell-specific differences in the post-translational processing of CgA and its peptide products. A panel of antisera that recognize specific domains of CgA may help to identify individual endocrine cell populations. (Less)
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chromogranin A, ECL cell, D cell, A-like cell, EC cell, G cell, processing
in
Journal of Histochemistry and Cytochemistry
volume
49
issue
1
pages
41535 - 41535
publisher
Histochemical Society
external identifiers
  • wos:000166045500002
  • scopus:0035187272
ISSN
0022-1554
language
English
LU publication?
yes
id
1217f3e3-bd2c-4ae5-8ecf-d58adc026e40 (old id 1119730)
alternative location
http://www.jhc.org/cgi/reprint/49/1/9
date added to LUP
2016-04-01 16:42:34
date last changed
2022-01-28 21:34:04
@article{1217f3e3-bd2c-4ae5-8ecf-d58adc026e40,
  abstract     = {{The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine Ib) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylsae (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and CgA-derived peptides in the various endocrine cell populations of the rat stomach. In the oxyntic mucosa, antisera raised against CgA(291-319) and CGA(316-321) immunostained D cells exclusively, whereas antisera raised against bCgA(82-91) and CgA(121-128) immunostained A-like cells and D cells. Antisera raised against CgA(318-349) and CgA(437-448) immunostained ECL cells and A-like cells, but not D cells. In the antrum, antisera against CgA(291-319) immunostained D cells, and antisera against CgA(351-356) immunostained G cells. Our observations suggest that each individual endocrine cell type in the rat stomach generates a unique mixture of CgA-derived peptides, probably reflecting cell-specific differences in the post-translational processing of CgA and its peptide products. A panel of antisera that recognize specific domains of CgA may help to identify individual endocrine cell populations.}},
  author       = {{Norlén, Per and Curry, William J. and Björkqvist, Maria and Maule, Aron and Cunningham, Rodat T. and Hogg, Robert B. and Harriott, Pat and Johnston, Colin F. and Hutton, John C. and Håkanson, Rolf}},
  issn         = {{0022-1554}},
  keywords     = {{chromogranin A; ECL cell; D cell; A-like cell; EC cell; G cell; processing}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{41535--41535}},
  publisher    = {{Histochemical Society}},
  series       = {{Journal of Histochemistry and Cytochemistry}},
  title        = {{Cell-specific processing of chromogranin A in endocrine cells of the rat stomach}},
  url          = {{http://www.jhc.org/cgi/reprint/49/1/9}},
  volume       = {{49}},
  year         = {{2001}},
}