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Alpha-1-antitrypsin is present in the specific granules of human eosinophilic granulocytes

Johansson, Björn LU ; Malm, Johan LU ; Persson, T; Janciauskiene, Sabina LU ; Andersson, P; Carlson, Joyce LU and Egesten, Arne LU (2001) In Clinical and Experimental Allergy 31(3). p.379-386
Abstract
Eosinophils may be found at sites of inflammation, for example in asthma, allergy and helminthic infestation, but their role in human inflammatory disease is unclear. In the present study, we investigated the presence of alpha-1-antitrypsin (AAT), a serine proteinase inhibitor, in human eosinophils. When lysates of highly purified eosinophils were subjected to Western blotting, with a chemiluminescent substrate, immunoreactive bands were seen. An ELISA was developed to measure the AAT content, which was found to be about 100 ng/5 x 106 eosinophils, about 50 ng/5 x 106 neutrophils, and about 25 ng/5 x 106 monocytes. Immunoelectron microscopy showed localization of AAT to the specific granules of eosinophils. During prolonged incubation of... (More)
Eosinophils may be found at sites of inflammation, for example in asthma, allergy and helminthic infestation, but their role in human inflammatory disease is unclear. In the present study, we investigated the presence of alpha-1-antitrypsin (AAT), a serine proteinase inhibitor, in human eosinophils. When lysates of highly purified eosinophils were subjected to Western blotting, with a chemiluminescent substrate, immunoreactive bands were seen. An ELISA was developed to measure the AAT content, which was found to be about 100 ng/5 x 106 eosinophils, about 50 ng/5 x 106 neutrophils, and about 25 ng/5 x 106 monocytes. Immunoelectron microscopy showed localization of AAT to the specific granules of eosinophils. During prolonged incubation of eosinophils, no significant increase in the total amount of AAT could be detected by ELISA. However, there was an increased level of AAT in the medium, in parallel with a decrease in the intracellular AAT content, suggesting release of preformed AAT. Apparent complex formation between iodinated elastase and AAT in eosinophil lysates provided evidence that the AAT is functionally active. On the basis of these findings it is suggested that by releasing AAT, eosinophils may, in a microenvironment, play a role in counteracting the tissue damage caused by serine proteinases released by neutrophils in inflammatory conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
granules, inflammation, eosinophils, alpha-1-antitrypsin, serpins, asthma
in
Clinical and Experimental Allergy
volume
31
issue
3
pages
379 - 386
publisher
Wiley-Blackwell
external identifiers
  • pmid:11260148
  • wos:000167851600007
  • scopus:0035723448
ISSN
1365-2222
DOI
language
English
LU publication?
yes
id
d9a9bf17-417b-4327-a02d-4598ae975aa9 (old id 1120016)
date added to LUP
2008-07-01 09:19:52
date last changed
2018-05-29 09:54:58
@article{d9a9bf17-417b-4327-a02d-4598ae975aa9,
  abstract     = {Eosinophils may be found at sites of inflammation, for example in asthma, allergy and helminthic infestation, but their role in human inflammatory disease is unclear. In the present study, we investigated the presence of alpha-1-antitrypsin (AAT), a serine proteinase inhibitor, in human eosinophils. When lysates of highly purified eosinophils were subjected to Western blotting, with a chemiluminescent substrate, immunoreactive bands were seen. An ELISA was developed to measure the AAT content, which was found to be about 100 ng/5 x 106 eosinophils, about 50 ng/5 x 106 neutrophils, and about 25 ng/5 x 106 monocytes. Immunoelectron microscopy showed localization of AAT to the specific granules of eosinophils. During prolonged incubation of eosinophils, no significant increase in the total amount of AAT could be detected by ELISA. However, there was an increased level of AAT in the medium, in parallel with a decrease in the intracellular AAT content, suggesting release of preformed AAT. Apparent complex formation between iodinated elastase and AAT in eosinophil lysates provided evidence that the AAT is functionally active. On the basis of these findings it is suggested that by releasing AAT, eosinophils may, in a microenvironment, play a role in counteracting the tissue damage caused by serine proteinases released by neutrophils in inflammatory conditions.},
  author       = {Johansson, Björn and Malm, Johan and Persson, T and Janciauskiene, Sabina and Andersson, P and Carlson, Joyce and Egesten, Arne},
  issn         = {1365-2222},
  keyword      = {granules,inflammation,eosinophils,alpha-1-antitrypsin,serpins,asthma},
  language     = {eng},
  number       = {3},
  pages        = {379--386},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical and Experimental Allergy},
  title        = {Alpha-1-antitrypsin is present in the specific granules of human eosinophilic granulocytes},
  url          = {http://dx.doi.org/},
  volume       = {31},
  year         = {2001},
}