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Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection

Zhang, Shu-min; Hui, Zhuang; Li, He-min; Qi, Zi-bai and Widell, Anders LU (2001) In Journal of Medical Virology 63(3). p.228-236
Abstract
Sequential serum samples from four plasma donors (designated A, B, C, and D) at a Chinese blood bank with hepatitis C transmission problems were studied from 1994 to 1997. The samples were examined for antibodies to HCV, for HCV viremia by PCR and HCV genotyping. Co- and superinfections were studied by direct sequencing of the 5'-NCR, core, and HVR-1 regions, using low and high genotype-specific primers targeting the HVR-1, and by cloning of selected samples. Genotype changes occurred in all four donors: A (1b-2a-1b), B (1b-2a-2a/1b-1b), C (1b-2a), and D (1b/2a-1b). Donor D was married to donor B. The 1b isolates of donor A could not be sequenced in the HVR-1 due to low-level viremia. Two early 1b isolates from donors B and C showed high... (More)
Sequential serum samples from four plasma donors (designated A, B, C, and D) at a Chinese blood bank with hepatitis C transmission problems were studied from 1994 to 1997. The samples were examined for antibodies to HCV, for HCV viremia by PCR and HCV genotyping. Co- and superinfections were studied by direct sequencing of the 5'-NCR, core, and HVR-1 regions, using low and high genotype-specific primers targeting the HVR-1, and by cloning of selected samples. Genotype changes occurred in all four donors: A (1b-2a-1b), B (1b-2a-2a/1b-1b), C (1b-2a), and D (1b/2a-1b). Donor D was married to donor B. The 1b isolates of donor A could not be sequenced in the HVR-1 due to low-level viremia. Two early 1b isolates from donors B and C showed high HVR-1 similarity. The later 1b isolates from B had changed significantly but were identical to the isolate from donor D. Spouses B and D also shared genotype 2a strains. The 2a isolates from donors A, B/D, and C differed by 8-10 nucleotides in the HVR-1. The frequent changes in genotype and the appearance of homologous isolates from different subjects indicate transmission at the blood bank. These four donors, all identified shortly after infection, developed very few mutations in the HVR-1 and few quasispecies during a period of 6-18 months. Highly specific primers proved to be superior to cloning for identification of minor virus populations. The results indicate nosocomial transmission of more than one strain at the blood bank studied. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hepatitis C virus, quasispecies, nosocomial infection, infected plasma donors, mutation rate
in
Journal of Medical Virology
volume
63
issue
3
pages
228 - 236
publisher
John Wiley & Sons
external identifiers
  • pmid:11170062
  • scopus:0035146075
ISSN
1096-9071
DOI
10.1002/1096-9071(200103)63:3<228::AID-JMV1005>3.0.CO;2-T
language
English
LU publication?
yes
id
65e9a0af-fe3b-446e-b51b-9d39af9dfd78 (old id 1120039)
date added to LUP
2008-07-18 16:28:16
date last changed
2018-05-29 10:13:37
@article{65e9a0af-fe3b-446e-b51b-9d39af9dfd78,
  abstract     = {Sequential serum samples from four plasma donors (designated A, B, C, and D) at a Chinese blood bank with hepatitis C transmission problems were studied from 1994 to 1997. The samples were examined for antibodies to HCV, for HCV viremia by PCR and HCV genotyping. Co- and superinfections were studied by direct sequencing of the 5'-NCR, core, and HVR-1 regions, using low and high genotype-specific primers targeting the HVR-1, and by cloning of selected samples. Genotype changes occurred in all four donors: A (1b-2a-1b), B (1b-2a-2a/1b-1b), C (1b-2a), and D (1b/2a-1b). Donor D was married to donor B. The 1b isolates of donor A could not be sequenced in the HVR-1 due to low-level viremia. Two early 1b isolates from donors B and C showed high HVR-1 similarity. The later 1b isolates from B had changed significantly but were identical to the isolate from donor D. Spouses B and D also shared genotype 2a strains. The 2a isolates from donors A, B/D, and C differed by 8-10 nucleotides in the HVR-1. The frequent changes in genotype and the appearance of homologous isolates from different subjects indicate transmission at the blood bank. These four donors, all identified shortly after infection, developed very few mutations in the HVR-1 and few quasispecies during a period of 6-18 months. Highly specific primers proved to be superior to cloning for identification of minor virus populations. The results indicate nosocomial transmission of more than one strain at the blood bank studied.},
  author       = {Zhang, Shu-min and Hui, Zhuang and Li, He-min and Qi, Zi-bai and Widell, Anders},
  issn         = {1096-9071},
  keyword      = {Hepatitis C virus,quasispecies,nosocomial infection,infected plasma donors,mutation rate},
  language     = {eng},
  number       = {3},
  pages        = {228--236},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Medical Virology},
  title        = {Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection},
  url          = {http://dx.doi.org/10.1002/1096-9071(200103)63:3<228::AID-JMV1005>3.0.CO;2-T},
  volume       = {63},
  year         = {2001},
}