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The tumor-associated gene HMGIC is expressed in normal and osteoarthritis-affected synovia

Broberg Palmgren, Karin LU ; Tallini, Giovanni; Höglund, Mattias LU ; Lindstrand, Anders LU ; Toksvig-Larsen, Sören LU and Mertens, Fredrik LU (2001) In Modern Pathology 14(4). p.311-317
Abstract
Chromosomal rearrangements involving chromosome bands 12q13-15 are very frequent findings in benign solid tumors, and recently, the primary molecular target for these aberrations was identified as the gene HMGIC. However, mutations in this gene have also been observed in nonneoplastic tissues. In a previous study, we reported breakpoints within HMGIC of synovia affected by osteoarthritis (OA) in two cases with 12q15 aberrations. To analyze further the role of HMGIC in this disease, we have performed cytogenetic, fluorescent in situ hybridization (FISH), RNA, and protein expression analyses on synovial samples from patients with OA and individuals without signs of the disorder. Cytogenetic analysis of short-term cultured cells revealed... (More)
Chromosomal rearrangements involving chromosome bands 12q13-15 are very frequent findings in benign solid tumors, and recently, the primary molecular target for these aberrations was identified as the gene HMGIC. However, mutations in this gene have also been observed in nonneoplastic tissues. In a previous study, we reported breakpoints within HMGIC of synovia affected by osteoarthritis (OA) in two cases with 12q15 aberrations. To analyze further the role of HMGIC in this disease, we have performed cytogenetic, fluorescent in situ hybridization (FISH), RNA, and protein expression analyses on synovial samples from patients with OA and individuals without signs of the disorder. Cytogenetic analysis of short-term cultured cells revealed clonal 12q13-15 aberrations in 2/36 cases of OA synovia and no rearrangement in any of the five controls. With FISH analysis, it was shown that the chromosomal breakpoints in the two aberrant cases were located outside the HMGIC locus. In contrast, at RNA and protein expression analyses, OA-affected as well as normal synovia displayed transcription and translation of the gene. We also analyzed whether immunoreactivity for HMGIC was associated with the proliferation-specific antigen Ki-67, but no correlation between the staining patterns of these proteins was observed. From the results of the present study, it is evident that expression of HMGIC cannot simply be considered a sign of neoplasia or an effect of proliferation. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Modern Pathology
volume
14
issue
4
pages
311 - 317
publisher
Nature Publishing Group
external identifiers
  • pmid:11301347
  • scopus:0035044743
ISSN
1530-0285
DOI
10.1038/modpathol.3880308
language
English
LU publication?
yes
id
8e59e21b-713d-49fb-8b14-1ac8a53800e5 (old id 1120108)
date added to LUP
2008-06-25 13:53:05
date last changed
2018-01-07 09:09:39
@article{8e59e21b-713d-49fb-8b14-1ac8a53800e5,
  abstract     = {Chromosomal rearrangements involving chromosome bands 12q13-15 are very frequent findings in benign solid tumors, and recently, the primary molecular target for these aberrations was identified as the gene HMGIC. However, mutations in this gene have also been observed in nonneoplastic tissues. In a previous study, we reported breakpoints within HMGIC of synovia affected by osteoarthritis (OA) in two cases with 12q15 aberrations. To analyze further the role of HMGIC in this disease, we have performed cytogenetic, fluorescent in situ hybridization (FISH), RNA, and protein expression analyses on synovial samples from patients with OA and individuals without signs of the disorder. Cytogenetic analysis of short-term cultured cells revealed clonal 12q13-15 aberrations in 2/36 cases of OA synovia and no rearrangement in any of the five controls. With FISH analysis, it was shown that the chromosomal breakpoints in the two aberrant cases were located outside the HMGIC locus. In contrast, at RNA and protein expression analyses, OA-affected as well as normal synovia displayed transcription and translation of the gene. We also analyzed whether immunoreactivity for HMGIC was associated with the proliferation-specific antigen Ki-67, but no correlation between the staining patterns of these proteins was observed. From the results of the present study, it is evident that expression of HMGIC cannot simply be considered a sign of neoplasia or an effect of proliferation.},
  author       = {Broberg Palmgren, Karin and Tallini, Giovanni and Höglund, Mattias and Lindstrand, Anders and Toksvig-Larsen, Sören and Mertens, Fredrik},
  issn         = {1530-0285},
  language     = {eng},
  number       = {4},
  pages        = {311--317},
  publisher    = {Nature Publishing Group},
  series       = {Modern Pathology},
  title        = {The tumor-associated gene HMGIC is expressed in normal and osteoarthritis-affected synovia},
  url          = {http://dx.doi.org/10.1038/modpathol.3880308},
  volume       = {14},
  year         = {2001},
}