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Stability of thiuram disulfides in patch test preparations and formation of asymmetric disulfides

Bergendorff, Ola LU and Hansson, Christer LU (2001) In Contact Dermatitis 45(3). p.151-157
Abstract
The thiuram mix used in patch testing originally contains 4 compounds. However, chemical analysis of the test preparation revealed that several new compounds are spontaneously formed during storage. The structures of these compounds have been determined and the rate of their formation has been studied in buffer solution at pH 7.4. After a few hours, a large amount of mixed disulfides are formed in solutions originally containing only symmetric disulfides. The impact on the test result of the formation of asymmetric disulfides has been investigated by testing on thiuram-sensitive volunteers with different preparations of mixed thiuram disulfides. In our study, the formation of new asymmetric thiuram disulfides from the original symmetric... (More)
The thiuram mix used in patch testing originally contains 4 compounds. However, chemical analysis of the test preparation revealed that several new compounds are spontaneously formed during storage. The structures of these compounds have been determined and the rate of their formation has been studied in buffer solution at pH 7.4. After a few hours, a large amount of mixed disulfides are formed in solutions originally containing only symmetric disulfides. The impact on the test result of the formation of asymmetric disulfides has been investigated by testing on thiuram-sensitive volunteers with different preparations of mixed thiuram disulfides. In our study, the formation of new asymmetric thiuram disulfides from the original symmetric thiuram disulfides in the test preparation had no influence on the result of the patch testing. However, as the chemical analysis showed that the mix composition changes during the period when the preparations are used, and differs between suppliers, the question is raised as to whether it is acceptable to use test preparations with a composition that is different from that labelled on the protocol. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Contact Dermatitis
volume
45
issue
3
pages
151 - 157
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:11553141
  • scopus:0034865739
ISSN
0105-1873
DOI
10.1034/j.1600-0536.2001.045003151.x
language
English
LU publication?
yes
id
47dbb11e-f909-431b-9a29-c27736b41039 (old id 1120255)
date added to LUP
2008-06-24 14:06:56
date last changed
2018-01-07 06:01:24
@article{47dbb11e-f909-431b-9a29-c27736b41039,
  abstract     = {The thiuram mix used in patch testing originally contains 4 compounds. However, chemical analysis of the test preparation revealed that several new compounds are spontaneously formed during storage. The structures of these compounds have been determined and the rate of their formation has been studied in buffer solution at pH 7.4. After a few hours, a large amount of mixed disulfides are formed in solutions originally containing only symmetric disulfides. The impact on the test result of the formation of asymmetric disulfides has been investigated by testing on thiuram-sensitive volunteers with different preparations of mixed thiuram disulfides. In our study, the formation of new asymmetric thiuram disulfides from the original symmetric thiuram disulfides in the test preparation had no influence on the result of the patch testing. However, as the chemical analysis showed that the mix composition changes during the period when the preparations are used, and differs between suppliers, the question is raised as to whether it is acceptable to use test preparations with a composition that is different from that labelled on the protocol.},
  author       = {Bergendorff, Ola and Hansson, Christer},
  issn         = {0105-1873},
  language     = {eng},
  number       = {3},
  pages        = {151--157},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Contact Dermatitis},
  title        = {Stability of thiuram disulfides in patch test preparations and formation of asymmetric disulfides},
  url          = {http://dx.doi.org/10.1034/j.1600-0536.2001.045003151.x},
  volume       = {45},
  year         = {2001},
}