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Different roles of spermine in glucocorticoid- and Fas-induced apoptosis

Hegardt, Cecilia LU ; Andersson, Gunnar and Oredsson, Stina LU (2001) In Experimental Cell Research 266(2). p.333-341
Abstract
Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both... (More)
Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glucocorticoid, apoptosis, Fas death receptor, thymocytes, Jurkat cells, sub-G1 peak, cell cycle phase distribution, polyamines, caspases, flow cytometry
in
Experimental Cell Research
volume
266
issue
2
pages
333 - 341
publisher
Academic Press
external identifiers
  • pmid:11399061
  • scopus:0034949763
ISSN
1090-2422
DOI
10.1006/excr.2001.5230
language
English
LU publication?
yes
id
7e34990a-62ec-42cd-a9a1-d1119cd1e118 (old id 1120285)
date added to LUP
2008-06-30 13:21:32
date last changed
2018-01-07 06:25:47
@article{7e34990a-62ec-42cd-a9a1-d1119cd1e118,
  abstract     = {Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation.},
  author       = {Hegardt, Cecilia and Andersson, Gunnar and Oredsson, Stina},
  issn         = {1090-2422},
  keyword      = {glucocorticoid,apoptosis,Fas death receptor,thymocytes,Jurkat cells,sub-G1 peak,cell cycle phase distribution,polyamines,caspases,flow cytometry},
  language     = {eng},
  number       = {2},
  pages        = {333--341},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Different roles of spermine in glucocorticoid- and Fas-induced apoptosis},
  url          = {http://dx.doi.org/10.1006/excr.2001.5230},
  volume       = {266},
  year         = {2001},
}