Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients
Roep, Bart O. ; Stobbe, Inge ; Duinkerken, Gaby ; Van Rood, Jon J. ; Lernmark, Åke LU
; Keymeulen, Bart
; Pipeleers, Danny
; Claas, Frans H.J.
and De Vries, René R.P.
(1999)
In Diabetes
48(3).
p.484-490
- Abstract
Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide- positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast,... (More)
Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide- positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast, rapid failure (<3 weeks) in three other cases was accompanied by increases in precursor frequencies of graft-specific alloreactive T-cells; in one of them, the alloreactivity was preceded by a sharply increased autoreactivity to several islet autoantigens. One recipient had a delayed loss of islet graft function (33 weeks); he did not exhibit signs of graft- specific alloimmunity, but developed a delayed increase in autoreactivity. The parallel between metabolic outcome of human β-cell allografts and cellular auto- and alloreactivity in peripheral blood suggests a causal relationship. The present study therefore demonstrates that T-cell reactivities in peripheral blood can be used to monitor immune mechanisms, which influence survival of β-cell allografts in diabetic patients.
(Less)
- author
- Roep, Bart O.
; Stobbe, Inge
; Duinkerken, Gaby
; Van Rood, Jon J.
; Lernmark, Åke
LU
; Keymeulen, Bart
; Pipeleers, Danny
; Claas, Frans H.J.
and De Vries, René R.P.
- publishing date
- 1999-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Diabetes
- volume
- 48
- issue
- 3
- pages
- 484 - 490
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- pmid:10078547
- scopus:0032971910
- ISSN
- 0012-1797
- DOI
- 10.2337/diabetes.48.3.484
- language
- English
- LU publication?
- no
- id
- 1120639d-edb4-46a8-ab1d-d4bbfffaf346
- date added to LUP
- 2019-06-30 23:31:22
- date last changed
- 2024-03-13 08:07:27
@article{1120639d-edb4-46a8-ab1d-d4bbfffaf346,
abstract = {{<p>Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide- positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast, rapid failure (<3 weeks) in three other cases was accompanied by increases in precursor frequencies of graft-specific alloreactive T-cells; in one of them, the alloreactivity was preceded by a sharply increased autoreactivity to several islet autoantigens. One recipient had a delayed loss of islet graft function (33 weeks); he did not exhibit signs of graft- specific alloimmunity, but developed a delayed increase in autoreactivity. The parallel between metabolic outcome of human β-cell allografts and cellular auto- and alloreactivity in peripheral blood suggests a causal relationship. The present study therefore demonstrates that T-cell reactivities in peripheral blood can be used to monitor immune mechanisms, which influence survival of β-cell allografts in diabetic patients.</p>}},
author = {{Roep, Bart O. and Stobbe, Inge and Duinkerken, Gaby and Van Rood, Jon J. and Lernmark, Åke and Keymeulen, Bart and Pipeleers, Danny and Claas, Frans H.J. and De Vries, René R.P.}},
issn = {{0012-1797}},
language = {{eng}},
month = {{01}},
number = {{3}},
pages = {{484--490}},
publisher = {{American Diabetes Association Inc.}},
series = {{Diabetes}},
title = {{Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients}},
url = {{http://dx.doi.org/10.2337/diabetes.48.3.484}},
doi = {{10.2337/diabetes.48.3.484}},
volume = {{48}},
year = {{1999}},
}