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Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo

Kadioglu, Aras; Sharpe, Jason A.; Lazou, Irene; Svanborg, Catharina LU ; Ockleford, Colin; Mitchell, Tim J. and Andrew, Peter W. (2001) In FEMS Microbiology Letters 194(1). p.105-110
Abstract
The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is... (More)
The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Letters
volume
194
issue
1
pages
105 - 110
publisher
Elsevier
external identifiers
  • pmid:11150674
  • scopus:0035163986
ISSN
1574-6968
DOI
10.1016/S0378-1097(00)00497-3
language
English
LU publication?
yes
id
588813cc-33c6-4504-936c-8f815129b831 (old id 1121121)
date added to LUP
2008-07-01 11:03:49
date last changed
2018-05-29 12:17:34
@article{588813cc-33c6-4504-936c-8f815129b831,
  abstract     = {The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.},
  author       = {Kadioglu, Aras and Sharpe, Jason A. and Lazou, Irene and Svanborg, Catharina and Ockleford, Colin and Mitchell, Tim J. and Andrew, Peter W.},
  issn         = {1574-6968},
  language     = {eng},
  number       = {1},
  pages        = {105--110},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo},
  url          = {http://dx.doi.org/10.1016/S0378-1097(00)00497-3},
  volume       = {194},
  year         = {2001},
}