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Ku protein in human T and B lymphocytes: full length functional form and signs of degradation

Sallmyr, Annahita LU ; Henriksson, Gunnel LU ; Fukushima, S and Bredberg, Anders LU (2001) In Biochimica et biophysica acta 1538(2-3). p.305-312
Abstract
DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent... (More)
DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ku protein, DNA-dependent protein kinase, B lymphocyte, T lymphocyte
in
Biochimica et biophysica acta
volume
1538
issue
2-3
pages
305 - 312
publisher
Elsevier
external identifiers
  • pmid:11336801
  • scopus:0035938186
ISSN
0006-3002
DOI
10.1016/S0167-4889(01)00081-7
language
English
LU publication?
yes
id
70154637-e2f0-4550-8ee5-e8c95af1d8c6 (old id 1121601)
date added to LUP
2008-07-14 09:27:29
date last changed
2018-10-03 11:58:14
@article{70154637-e2f0-4550-8ee5-e8c95af1d8c6,
  abstract     = {DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa.},
  author       = {Sallmyr, Annahita and Henriksson, Gunnel and Fukushima, S and Bredberg, Anders},
  issn         = {0006-3002},
  keyword      = {Ku protein,DNA-dependent protein kinase,B lymphocyte,T lymphocyte},
  language     = {eng},
  number       = {2-3},
  pages        = {305--312},
  publisher    = {Elsevier},
  series       = {Biochimica et biophysica acta},
  title        = {Ku protein in human T and B lymphocytes: full length functional form and signs of degradation},
  url          = {http://dx.doi.org/10.1016/S0167-4889(01)00081-7},
  volume       = {1538},
  year         = {2001},
}