Ku protein in human T and B lymphocytes: full length functional form and signs of degradation
(2001) In Biochimica et Biophysica Acta 1538(2-3). p.305-312- Abstract
- DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent... (More)
- DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121601
- author
- Sallmyr, Annahita LU ; Henriksson, Gunnel LU ; Fukushima, S and Bredberg, Anders LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ku protein, DNA-dependent protein kinase, B lymphocyte, T lymphocyte
- in
- Biochimica et Biophysica Acta
- volume
- 1538
- issue
- 2-3
- pages
- 305 - 312
- publisher
- Elsevier
- external identifiers
-
- pmid:11336801
- scopus:0035938186
- ISSN
- 0006-3002
- DOI
- 10.1016/S0167-4889(01)00081-7
- language
- English
- LU publication?
- yes
- id
- 70154637-e2f0-4550-8ee5-e8c95af1d8c6 (old id 1121601)
- date added to LUP
- 2016-04-01 15:54:11
- date last changed
- 2022-01-28 07:55:03
@article{70154637-e2f0-4550-8ee5-e8c95af1d8c6, abstract = {{DNA-dependent protein kinase (DNA-PK) has been shown to take part in cell cycle regulatory signal transduction and in the repair of X-ray-induced DNA double-strand breaks. Functional DNA-PK is furthermore needed for the generation of antigen specificity during lymphocyte maturation. The Ku86 subunit of DNA-PK has been reported to exist in human B lymphocytes in a truncated form capable of binding to broken DNA but lacking the ability to activate the kinase function of DNA-PK. In the present work the Ku70 and Ku86 dimer proteins in T and B lymphocytes from human blood donors were analysed by immunoblotting and were observed apparently to be of full length. Also, nuclear protein extracted from B and non-B lymphocytes displayed DNA-dependent kinase activity. However, a minor fraction of Ku86 in lymphocytes was observed to be truncated with a molecular mass of approx. 70 kDa.}}, author = {{Sallmyr, Annahita and Henriksson, Gunnel and Fukushima, S and Bredberg, Anders}}, issn = {{0006-3002}}, keywords = {{Ku protein; DNA-dependent protein kinase; B lymphocyte; T lymphocyte}}, language = {{eng}}, number = {{2-3}}, pages = {{305--312}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta}}, title = {{Ku protein in human T and B lymphocytes: full length functional form and signs of degradation}}, url = {{http://dx.doi.org/10.1016/S0167-4889(01)00081-7}}, doi = {{10.1016/S0167-4889(01)00081-7}}, volume = {{1538}}, year = {{2001}}, }