Advanced

Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitol

Hultberg, Björn LU ; Andersson, Anders and Isaksson, Anders LU (2001) In Toxicology 156(2-3). p.93-100
Abstract
In the present study, we have investigated the effects of extracellular redox status and metal/thiol interactions on glutathione distribution in HeLa cell cultures. No effects were seen on glutathione distribution after the addition of different thiols, whereas the pro-oxidant copper ions affected glutathione distribution in several ways. The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell toxicity. In the presence of DTT, increased intra- and extracellular glutathione concentrations were noted already at 0.05 micromol/l, which was below the previously reported toxicity threshold for mercury ions in blood. Likewise DTT potentiated the effects of copper... (More)
In the present study, we have investigated the effects of extracellular redox status and metal/thiol interactions on glutathione distribution in HeLa cell cultures. No effects were seen on glutathione distribution after the addition of different thiols, whereas the pro-oxidant copper ions affected glutathione distribution in several ways. The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell toxicity. In the presence of DTT, increased intra- and extracellular glutathione concentrations were noted already at 0.05 micromol/l, which was below the previously reported toxicity threshold for mercury ions in blood. Likewise DTT potentiated the effects of copper ions on glutathione distribution and cell toxicity, whereas the addition of DTT to cell cultures with a non-metal thiol reactive agent (hydroquinone) or an oxidative agent (hydrogen peroxide) did not affect glutathione distribution or cell toxicity. Thus, it seems as the synergistic effects between DTT and thiol reactive agents only apply to metal ions. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell culture, Copper ions, Dithiothreitol, Glutathione, Homocysteine, Hydrogen peroxide, Hydroquinone, Mercury ions, N-acetylcysteine
in
Toxicology
volume
156
issue
2-3
pages
93 - 100
publisher
Elsevier
external identifiers
  • pmid:11164611
  • scopus:0035793128
ISSN
0300-483X
DOI
10.1016/S0300-483X(00)00331-0
language
English
LU publication?
yes
id
b1f6363a-cc59-4c29-bee5-331322a4c67d (old id 1122322)
date added to LUP
2008-06-30 15:43:55
date last changed
2018-02-04 03:56:23
@article{b1f6363a-cc59-4c29-bee5-331322a4c67d,
  abstract     = {In the present study, we have investigated the effects of extracellular redox status and metal/thiol interactions on glutathione distribution in HeLa cell cultures. No effects were seen on glutathione distribution after the addition of different thiols, whereas the pro-oxidant copper ions affected glutathione distribution in several ways. The addition of dithiothreitol (DTT) but not the other thiols potentiated the effects of mercury ions on glutathione distribution and cell toxicity. In the presence of DTT, increased intra- and extracellular glutathione concentrations were noted already at 0.05 micromol/l, which was below the previously reported toxicity threshold for mercury ions in blood. Likewise DTT potentiated the effects of copper ions on glutathione distribution and cell toxicity, whereas the addition of DTT to cell cultures with a non-metal thiol reactive agent (hydroquinone) or an oxidative agent (hydrogen peroxide) did not affect glutathione distribution or cell toxicity. Thus, it seems as the synergistic effects between DTT and thiol reactive agents only apply to metal ions.},
  author       = {Hultberg, Björn and Andersson, Anders and Isaksson, Anders},
  issn         = {0300-483X},
  keyword      = {Cell culture,Copper ions,Dithiothreitol,Glutathione,Homocysteine,Hydrogen peroxide,Hydroquinone,Mercury ions,N-acetylcysteine},
  language     = {eng},
  number       = {2-3},
  pages        = {93--100},
  publisher    = {Elsevier},
  series       = {Toxicology},
  title        = {Interaction of metals and thiols in cell damage and glutathione distribution: potentiation of mercury toxicity by dithiothreitol},
  url          = {http://dx.doi.org/10.1016/S0300-483X(00)00331-0},
  volume       = {156},
  year         = {2001},
}