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Development of wide-pore CLEAR supports for applications involving biological macromolecules.

Cederfur, Josefine LU and Kempe, Maria LU (2001) In Polymer Bulletin 46(5). p.381-387
Abstract
The CLEAR supports were originally developed for solid-phase synthesis. In this study, two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen. Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent. Resins of two different porosities were synthesized. The permeability to biological macromolecules was investigated by size exclusion chromatography, using the resin beads as the stationary phase. One of the resins was able to retard thyroglobulin (669 kDa) while the other, prepared with a lower amount of porogen, showed a lower fractionation range (1-60 kDa).
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Polymer Bulletin
volume
46
issue
5
pages
381 - 387
publisher
Springer
external identifiers
  • scopus:0034881044
ISSN
0170-0839
DOI
10.1007/s002890170046
language
English
LU publication?
yes
id
009387dd-f252-4c7b-a86e-659d1b190797 (old id 1122670)
date added to LUP
2008-06-25 16:07:29
date last changed
2018-05-29 12:21:55
@article{009387dd-f252-4c7b-a86e-659d1b190797,
  abstract     = {The CLEAR supports were originally developed for solid-phase synthesis. In this study, two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen. Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent. Resins of two different porosities were synthesized. The permeability to biological macromolecules was investigated by size exclusion chromatography, using the resin beads as the stationary phase. One of the resins was able to retard thyroglobulin (669 kDa) while the other, prepared with a lower amount of porogen, showed a lower fractionation range (1-60 kDa).},
  author       = {Cederfur, Josefine and Kempe, Maria},
  issn         = {0170-0839},
  language     = {eng},
  number       = {5},
  pages        = {381--387},
  publisher    = {Springer},
  series       = {Polymer Bulletin},
  title        = {Development of wide-pore CLEAR supports for applications involving biological macromolecules.},
  url          = {http://dx.doi.org/10.1007/s002890170046},
  volume       = {46},
  year         = {2001},
}