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Evolution of DNA ploidy during squamous cell carcinogenesis in the esophagus

Blant, S A; Ballini, J-P; Caron, C T; Fontolliet, C; Monnier, P and Laurini, Ricardo LU (2001) In Diseases of the Esophagus 14(3-4). p.178-184
Abstract
Image and flow cytometry was used to study the nuclear DNA content (ploidy) during the squamous cell carcinogenesis in the esophagus. The present retrospective study comprised 26 surgical specimens of squamous cell carcinomas (SCC) in patients who underwent surgery alone at the Department of Surgery in CHUV Hospital in Lausanne, between January 1992 and December 1999. We analyzed 53 healthy tissues, 43 tumors, and six lymph node metastases. Diploid DNA histogram patterns were observed in all non-pathologic tissues analyzed, either distant or proximal to the lesion. Aneuploidy was observed in 30 (70%) of 43 lesions; 20 (62.5%) of 32 early squamous-cell carcinomas; and 10 (91%) of 11 advanced carcinomas. In patients with various tumor stages... (More)
Image and flow cytometry was used to study the nuclear DNA content (ploidy) during the squamous cell carcinogenesis in the esophagus. The present retrospective study comprised 26 surgical specimens of squamous cell carcinomas (SCC) in patients who underwent surgery alone at the Department of Surgery in CHUV Hospital in Lausanne, between January 1992 and December 1999. We analyzed 53 healthy tissues, 43 tumors, and six lymph node metastases. Diploid DNA histogram patterns were observed in all non-pathologic tissues analyzed, either distant or proximal to the lesion. Aneuploidy was observed in 30 (70%) of 43 lesions; 20 (62.5%) of 32 early squamous-cell carcinomas; and 10 (91%) of 11 advanced carcinomas. In patients with various tumor stages or with multicentric synchronous or metachronous tumors, DNA content was not different among different tumor stages. Four of six lymph node metastases had the same DNA content as the primary tumor. In four patients, discordance between image and flow cytometry analysis was observed for malignant lesions only. Ploidy status was not statistically associated with the differentiation of the tumor, but it was associated with the stage of tumor (P < 0.001). These findings suggest that early malignant changes in the esophagus are already associated with alteration in DNA content, and aneuploidy tends to correlate with progression to invasive SCC. This cell kinetic information could help clinicians in selecting the optimal treatment for the individual patient. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diseases of the Esophagus
volume
14
issue
3-4
pages
178 - 184
publisher
Wiley-Blackwell
external identifiers
  • pmid:11869316
  • scopus:0035716114
ISSN
1120-8694
DOI
10.1046/j.1442-2050.2001.00182.x
language
English
LU publication?
yes
id
6c353e2c-e6bf-4404-9f9e-a088fd378025 (old id 1122983)
date added to LUP
2008-08-28 09:48:15
date last changed
2018-05-29 09:30:59
@article{6c353e2c-e6bf-4404-9f9e-a088fd378025,
  abstract     = {Image and flow cytometry was used to study the nuclear DNA content (ploidy) during the squamous cell carcinogenesis in the esophagus. The present retrospective study comprised 26 surgical specimens of squamous cell carcinomas (SCC) in patients who underwent surgery alone at the Department of Surgery in CHUV Hospital in Lausanne, between January 1992 and December 1999. We analyzed 53 healthy tissues, 43 tumors, and six lymph node metastases. Diploid DNA histogram patterns were observed in all non-pathologic tissues analyzed, either distant or proximal to the lesion. Aneuploidy was observed in 30 (70%) of 43 lesions; 20 (62.5%) of 32 early squamous-cell carcinomas; and 10 (91%) of 11 advanced carcinomas. In patients with various tumor stages or with multicentric synchronous or metachronous tumors, DNA content was not different among different tumor stages. Four of six lymph node metastases had the same DNA content as the primary tumor. In four patients, discordance between image and flow cytometry analysis was observed for malignant lesions only. Ploidy status was not statistically associated with the differentiation of the tumor, but it was associated with the stage of tumor (P &lt; 0.001). These findings suggest that early malignant changes in the esophagus are already associated with alteration in DNA content, and aneuploidy tends to correlate with progression to invasive SCC. This cell kinetic information could help clinicians in selecting the optimal treatment for the individual patient.},
  author       = {Blant, S A and Ballini, J-P and Caron, C T and Fontolliet, C and Monnier, P and Laurini, Ricardo},
  issn         = {1120-8694},
  language     = {eng},
  number       = {3-4},
  pages        = {178--184},
  publisher    = {Wiley-Blackwell},
  series       = {Diseases of the Esophagus},
  title        = {Evolution of DNA ploidy during squamous cell carcinogenesis in the esophagus},
  url          = {http://dx.doi.org/10.1046/j.1442-2050.2001.00182.x},
  volume       = {14},
  year         = {2001},
}