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Defensin-rich granules of human neutrophils: characterization of secretory properties

Faurschou, Mikkel; Sørensen, Ole E LU ; Johnsen, Anders H; Askaa, Jon and Borregaard, Niels (2002) In Biochimica et Biophysica Acta 1591(1-3). p.29-35
Abstract
The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil... (More)
The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil (myeloperoxidase), specific (lactoferrin) and gelatinase (gelatinase) granules from neutrophils stimulated with different secretagogues. The release pattern of alpha-defensins correlated perfectly with the release of myeloperoxidase and showed no resemblance to the exocytosis of lactoferrin or gelatinase. This finding was substantiated through subcellular fractionation experiments. In conclusion, despite a distinct profile of biosynthesis, DRG are indistinguishable from defensin-poor azurophil granules with respect to exocytosis. Thus, in contrast to peroxidase-negative granules, azurophil granules display homogeneity in their availability for extracellular release. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta
volume
1591
issue
1-3
pages
29 - 35
publisher
Elsevier
external identifiers
  • pmid:12183052
  • scopus:0037135707
ISSN
0006-3002
DOI
10.1016/S0167-4889(02)00243-4
language
English
LU publication?
no
id
4270e568-76ce-451e-bad7-da251006ca6f (old id 1123808)
date added to LUP
2008-05-21 10:10:24
date last changed
2017-10-22 04:30:09
@article{4270e568-76ce-451e-bad7-da251006ca6f,
  abstract     = {The various granule subtypes of the human neutrophil differ in propensity for exocytosis. As a rule, granules formed at late stages of myelopoiesis have a higher secretory potential than granules formed in more immature myeloid cells. Neutrophils contain four closely related alpha-defensins, which are stored in a subset of azurophil granules. These defensin-rich azurophil granules (DRG) are formed later than defensin-poor azurophil granules, near the promyelocyte/myelocyte transition. In order to characterize the secretory properties of DRG, we developed a sensitive and accurate ELISA for detection of the neutrophil alpha-defensins HNP 1-3. This allowed us to quantify the exocytosis of alpha-defensins and markers of azurophil (myeloperoxidase), specific (lactoferrin) and gelatinase (gelatinase) granules from neutrophils stimulated with different secretagogues. The release pattern of alpha-defensins correlated perfectly with the release of myeloperoxidase and showed no resemblance to the exocytosis of lactoferrin or gelatinase. This finding was substantiated through subcellular fractionation experiments. In conclusion, despite a distinct profile of biosynthesis, DRG are indistinguishable from defensin-poor azurophil granules with respect to exocytosis. Thus, in contrast to peroxidase-negative granules, azurophil granules display homogeneity in their availability for extracellular release.},
  author       = {Faurschou, Mikkel and Sørensen, Ole E and Johnsen, Anders H and Askaa, Jon and Borregaard, Niels},
  issn         = {0006-3002},
  language     = {eng},
  number       = {1-3},
  pages        = {29--35},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta},
  title        = {Defensin-rich granules of human neutrophils: characterization of secretory properties},
  url          = {http://dx.doi.org/10.1016/S0167-4889(02)00243-4},
  volume       = {1591},
  year         = {2002},
}