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Stable GAD65 autoantibody epitope patterns in type 1 diabetes children five years after onset.

Hampe, CS; Hammerle, LP; Bekris, L; Ortqvist, E; Persson, B and Lernmark, Åke LU (2002) In Journal of Autoimmunity 18(1). p.49-53
Abstract
Autoantibodies to GAD65 (GAD65Ab) are prominent in type 1 diabetes. These autoantibodies may be present both years before and after the clinical diagnosis of type 1 diabetes and are widely used as a marker for the disease. Recently it has been demonstrated that progression to type 1 diabetes is accompanied by GAD65Ab epitope maturation. Here we examine whether autoantibody maturation processes also progress after the clinical diagnosis of type 1 diabetes. Antibody reactivity to GAD65, GAD67 and GAD65/67 fusion proteins was measured by radioimmunoassays in 62 children with type 1 diabetes. Samples were taken at diagnosis and five years later. While the overall GAD65Ab level declined over time, the epitope pattern was remarkably stable with... (More)
Autoantibodies to GAD65 (GAD65Ab) are prominent in type 1 diabetes. These autoantibodies may be present both years before and after the clinical diagnosis of type 1 diabetes and are widely used as a marker for the disease. Recently it has been demonstrated that progression to type 1 diabetes is accompanied by GAD65Ab epitope maturation. Here we examine whether autoantibody maturation processes also progress after the clinical diagnosis of type 1 diabetes. Antibody reactivity to GAD65, GAD67 and GAD65/67 fusion proteins was measured by radioimmunoassays in 62 children with type 1 diabetes. Samples were taken at diagnosis and five years later. While the overall GAD65Ab level declined over time, the epitope pattern was remarkably stable with no significant changes in binding pattern. Loss of GAD65Ab-positivity was associated with significantly lower GAD65Ab indices at diagnosis compared to patients' sera that remained GAD65Ab-positive. The decrease in GAD65Ab levels did not correlate to r (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Autoimmunity
volume
18
issue
1
pages
49 - 53
publisher
Elsevier
external identifiers
  • Scopus:0037006434
ISSN
0896-8411
DOI
10.1006/jaut.2001.0564
language
English
LU publication?
no
id
3815743a-2c59-4c71-b7ff-f24af2d08a9e (old id 1124232)
date added to LUP
2008-05-22 13:56:18
date last changed
2017-01-01 07:56:52
@article{3815743a-2c59-4c71-b7ff-f24af2d08a9e,
  abstract     = {Autoantibodies to GAD65 (GAD65Ab) are prominent in type 1 diabetes. These autoantibodies may be present both years before and after the clinical diagnosis of type 1 diabetes and are widely used as a marker for the disease. Recently it has been demonstrated that progression to type 1 diabetes is accompanied by GAD65Ab epitope maturation. Here we examine whether autoantibody maturation processes also progress after the clinical diagnosis of type 1 diabetes. Antibody reactivity to GAD65, GAD67 and GAD65/67 fusion proteins was measured by radioimmunoassays in 62 children with type 1 diabetes. Samples were taken at diagnosis and five years later. While the overall GAD65Ab level declined over time, the epitope pattern was remarkably stable with no significant changes in binding pattern. Loss of GAD65Ab-positivity was associated with significantly lower GAD65Ab indices at diagnosis compared to patients' sera that remained GAD65Ab-positive. The decrease in GAD65Ab levels did not correlate to r},
  author       = {Hampe, CS and Hammerle, LP and Bekris, L and Ortqvist, E and Persson, B and Lernmark, Åke},
  issn         = {0896-8411},
  language     = {eng},
  number       = {1},
  pages        = {49--53},
  publisher    = {Elsevier},
  series       = {Journal of Autoimmunity},
  title        = {Stable GAD65 autoantibody epitope patterns in type 1 diabetes children five years after onset.},
  url          = {http://dx.doi.org/10.1006/jaut.2001.0564},
  volume       = {18},
  year         = {2002},
}