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Spermine prevents cytochrome c release in glucocorticoid-induced apoptosis in mouse thymocytes.

Hegardt, Cecilia LU ; Andersson, Gunnar and Oredsson, Stina LU (2003) In Cell Biology International 27(2). p.115-121
Abstract
Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen... (More)
Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen concanavalin A (Con A) was added to dexamethasone- plus spermine-treated thymocytes, the number of apoptotic cells in the pre-G1peak was reduced compared to thymocytes treated with only dexamethasone plus spermine. Comparing concanavalin A added to dexamethasone-treated or to dexamethasone plus spermine-treated thymocytes, showed a markedly reduced pre-G1peak in the latter. Thus, the spermine-induced inhibition of cytochrome c release confers a survival advantage on thymocytes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Biology International
volume
27
issue
2
pages
115 - 121
publisher
Elsevier
external identifiers
  • wos:000181700300004
  • pmid:12662968
  • scopus:0037299620
ISSN
1095-8355
DOI
10.1016/S1065-6995(02)00291-3
language
English
LU publication?
yes
id
ff4dc913-41bd-4865-8f44-d48c32e5f7b0 (old id 112464)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12662968
date added to LUP
2007-07-18 16:36:59
date last changed
2018-05-29 09:44:58
@article{ff4dc913-41bd-4865-8f44-d48c32e5f7b0,
  abstract     = {Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen concanavalin A (Con A) was added to dexamethasone- plus spermine-treated thymocytes, the number of apoptotic cells in the pre-G1peak was reduced compared to thymocytes treated with only dexamethasone plus spermine. Comparing concanavalin A added to dexamethasone-treated or to dexamethasone plus spermine-treated thymocytes, showed a markedly reduced pre-G1peak in the latter. Thus, the spermine-induced inhibition of cytochrome c release confers a survival advantage on thymocytes.},
  author       = {Hegardt, Cecilia and Andersson, Gunnar and Oredsson, Stina},
  issn         = {1095-8355},
  language     = {eng},
  number       = {2},
  pages        = {115--121},
  publisher    = {Elsevier},
  series       = {Cell Biology International},
  title        = {Spermine prevents cytochrome c release in glucocorticoid-induced apoptosis in mouse thymocytes.},
  url          = {http://dx.doi.org/10.1016/S1065-6995(02)00291-3},
  volume       = {27},
  year         = {2003},
}