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GRACILE syndrome, a lethal metabolic disorder with iron overload, is caused by a point mutation in BCS1L

Visapaa, Ilona ; Fellman, Vineta LU orcid ; Vesa, Jouni ; Dasvarma, Ayan ; Hutton, Jenna L ; Kumar, Vijay ; Payne, Gregory S ; Makarow, Marja ; Van Coster, Rudy and Taylor, Robert W , et al. (2002) In American Journal of Human Genetics 71(4). p.863-876
Abstract
GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome is a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism. We previously localized the causative gene to a 1.5-cM region on chromosome 2q33-37. In the present study, we report the molecular defect causing this metabolic disorder, by identifying a homozygous missense mutation that results in an S78G amino acid change in the BCS1L gene in Finnish patients with GRACILE syndrome, as well as five different mutations in three British infants. BCS1L, a mitochondrial inner-membrane protein, is a chaperone necessary for the... (More)
GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome is a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism. We previously localized the causative gene to a 1.5-cM region on chromosome 2q33-37. In the present study, we report the molecular defect causing this metabolic disorder, by identifying a homozygous missense mutation that results in an S78G amino acid change in the BCS1L gene in Finnish patients with GRACILE syndrome, as well as five different mutations in three British infants. BCS1L, a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Pulse-chase experiments performed in COS-1 cells indicated that the S78G amino acid change results in instability of the polypeptide, and yeast complementation studies revealed a functional defect in the mutated BCS1L protein. Four different mutations in the BCS1L gene have been reported elsewhere, in Turkish patients with a distinctly different phenotype. Interestingly, the British and Turkish patients had complex III deficiency, whereas in the Finnish patients with GRACILE syndrome complex III activity was within the normal range, implying that BCS1L has another cellular function that is uncharacterized but essential and is putatively involved in iron metabolism. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Human Genetics
volume
71
issue
4
pages
863 - 876
publisher
Cell Press
external identifiers
  • pmid:12215968
  • scopus:19044365959
ISSN
0002-9297
DOI
10.1086/342773
language
English
LU publication?
no
id
a8617ec5-d2e1-4137-8159-5779d82e8d14 (old id 1124757)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12215968
date added to LUP
2016-04-01 11:45:48
date last changed
2022-04-13 00:51:52
@article{a8617ec5-d2e1-4137-8159-5779d82e8d14,
  abstract     = {{GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome is a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism. We previously localized the causative gene to a 1.5-cM region on chromosome 2q33-37. In the present study, we report the molecular defect causing this metabolic disorder, by identifying a homozygous missense mutation that results in an S78G amino acid change in the BCS1L gene in Finnish patients with GRACILE syndrome, as well as five different mutations in three British infants. BCS1L, a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Pulse-chase experiments performed in COS-1 cells indicated that the S78G amino acid change results in instability of the polypeptide, and yeast complementation studies revealed a functional defect in the mutated BCS1L protein. Four different mutations in the BCS1L gene have been reported elsewhere, in Turkish patients with a distinctly different phenotype. Interestingly, the British and Turkish patients had complex III deficiency, whereas in the Finnish patients with GRACILE syndrome complex III activity was within the normal range, implying that BCS1L has another cellular function that is uncharacterized but essential and is putatively involved in iron metabolism.}},
  author       = {{Visapaa, Ilona and Fellman, Vineta and Vesa, Jouni and Dasvarma, Ayan and Hutton, Jenna L and Kumar, Vijay and Payne, Gregory S and Makarow, Marja and Van Coster, Rudy and Taylor, Robert W and Turnbull, Douglass M and Suomalainen, Anu and Peltonen, Leena}},
  issn         = {{0002-9297}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{863--876}},
  publisher    = {{Cell Press}},
  series       = {{American Journal of Human Genetics}},
  title        = {{GRACILE syndrome, a lethal metabolic disorder with iron overload, is caused by a point mutation in BCS1L}},
  url          = {{http://dx.doi.org/10.1086/342773}},
  doi          = {{10.1086/342773}},
  volume       = {{71}},
  year         = {{2002}},
}