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Recombinant human albumin as protein source in culture media used for IVF: a prospective randomized study

Bungum, Mona LU ; Humaidan, P and Bungum, Leif LU (2002) In Reproductive BioMedicine Online 4(3). p.233-236
Abstract
Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocyst formation and implantation were observed in both groups. The frequency of pregnancy and early pregnancy loss were also equal. It is concluded that culture media containing rHA seem to produce embryos of high quality comparable to media containing HSA. Therefore, rHA may replace HSA as a protein source in culture media for IVF and may reduce... (More)
Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocyst formation and implantation were observed in both groups. The frequency of pregnancy and early pregnancy loss were also equal. It is concluded that culture media containing rHA seem to produce embryos of high quality comparable to media containing HSA. Therefore, rHA may replace HSA as a protein source in culture media for IVF and may reduce risks of prion contamination and transmission of plasma derived impurities. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Reproductive BioMedicine Online
volume
4
issue
3
pages
233 - 236
publisher
Reproductive Healthcare Ltd
external identifiers
  • pmid:12709272
  • scopus:0038050401
ISSN
1472-6491
language
English
LU publication?
no
id
193524f4-9753-4a24-bcd0-ced9ad12d6c4 (old id 1124786)
alternative location
http://www.ingentaconnect.com/content/repro/rebi/2002/00000004/00000003/art00006
date added to LUP
2008-05-20 12:14:49
date last changed
2017-10-29 03:32:54
@article{193524f4-9753-4a24-bcd0-ced9ad12d6c4,
  abstract     = {Oocytes obtained from 85 women undergoing IVF/intracytoplasmic sperm injection (ICSI) treatment were randomly selected for culture in media containing either human serum albumin (HSA), (n = 42) or recombinant human albumin (rHA), (n = 43). At the time of transfer, the two embryos with the overall best morphology were selected on day 2, 3 or 5. Comparable rates of fertilization, cleavage, blastocyst formation and implantation were observed in both groups. The frequency of pregnancy and early pregnancy loss were also equal. It is concluded that culture media containing rHA seem to produce embryos of high quality comparable to media containing HSA. Therefore, rHA may replace HSA as a protein source in culture media for IVF and may reduce risks of prion contamination and transmission of plasma derived impurities.},
  author       = {Bungum, Mona and Humaidan, P and Bungum, Leif},
  issn         = {1472-6491},
  language     = {eng},
  number       = {3},
  pages        = {233--236},
  publisher    = {Reproductive Healthcare Ltd},
  series       = {Reproductive BioMedicine Online},
  title        = {Recombinant human albumin as protein source in culture media used for IVF: a prospective randomized study},
  volume       = {4},
  year         = {2002},
}