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Streptozotocin induced diabetes in minipig: a case report of a possible model for type 1 diabetes?

Rolandsson, O; Haney, M F; Hagg, E; Biber, B and Lernmark, Åke LU (2002) In Autoimmunity 35(4). p.261-264
Abstract
Studies on the pathogenic process in type 1 diabetes are often performed in animal models. Low-dose administration of streptozotocin has been used to induce diabetes with pathological alterations similar to human type 1 diabetes in the animals. Rodent models are frequently used but there is a need of developing new models including larger animals. In this study we wanted to investigate to what extent a minipig was sensitive to low-dose streptozotocin for induction of diabetes with features of human Type I diabetes. A female Göttingen minipig received two low-doses (40 mg/kg) of streptozotocin with an 11-day interval. Serum was analysed for the presence of the enzyme glutamic acid decarboxylase, isoform 65, (GAD65) and autoantibodies... (More)
Studies on the pathogenic process in type 1 diabetes are often performed in animal models. Low-dose administration of streptozotocin has been used to induce diabetes with pathological alterations similar to human type 1 diabetes in the animals. Rodent models are frequently used but there is a need of developing new models including larger animals. In this study we wanted to investigate to what extent a minipig was sensitive to low-dose streptozotocin for induction of diabetes with features of human Type I diabetes. A female Göttingen minipig received two low-doses (40 mg/kg) of streptozotocin with an 11-day interval. Serum was analysed for the presence of the enzyme glutamic acid decarboxylase, isoform 65, (GAD65) and autoantibodies against glutamic acid decarboxylase, isoform 65 (GAD65A), isoform 67 (GAD67A), insulinoma antigen 2 (IA-2) and insulin (IAA). Pancreas tissue was fixated in formaldehyde and was sent for pathoanatomical examination. The minipig became hyperglycaemic after t (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Autoimmunity
volume
35
issue
4
pages
261 - 264
publisher
Taylor & Francis
external identifiers
  • scopus:0035995042
ISSN
0891-6934
DOI
10.1080/0891693021000010703
language
English
LU publication?
no
id
1e0f0b82-7415-49c6-a18e-d6b4ae74d9bd (old id 1124789)
date added to LUP
2008-05-30 07:45:39
date last changed
2017-11-12 03:23:52
@article{1e0f0b82-7415-49c6-a18e-d6b4ae74d9bd,
  abstract     = {Studies on the pathogenic process in type 1 diabetes are often performed in animal models. Low-dose administration of streptozotocin has been used to induce diabetes with pathological alterations similar to human type 1 diabetes in the animals. Rodent models are frequently used but there is a need of developing new models including larger animals. In this study we wanted to investigate to what extent a minipig was sensitive to low-dose streptozotocin for induction of diabetes with features of human Type I diabetes. A female Göttingen minipig received two low-doses (40 mg/kg) of streptozotocin with an 11-day interval. Serum was analysed for the presence of the enzyme glutamic acid decarboxylase, isoform 65, (GAD65) and autoantibodies against glutamic acid decarboxylase, isoform 65 (GAD65A), isoform 67 (GAD67A), insulinoma antigen 2 (IA-2) and insulin (IAA). Pancreas tissue was fixated in formaldehyde and was sent for pathoanatomical examination. The minipig became hyperglycaemic after t},
  author       = {Rolandsson, O and Haney, M F and Hagg, E and Biber, B and Lernmark, Åke},
  issn         = {0891-6934},
  language     = {eng},
  number       = {4},
  pages        = {261--264},
  publisher    = {Taylor & Francis},
  series       = {Autoimmunity},
  title        = {Streptozotocin induced diabetes in minipig: a case report of a possible model for type 1 diabetes?},
  url          = {http://dx.doi.org/10.1080/0891693021000010703},
  volume       = {35},
  year         = {2002},
}