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Dynamic perifusion to maintain and assess isolated pancreatic islets.

Sweet, I R; Cook, D L; Wiseman, R W; Greenbaum, C J; Lernmark, Åke LU ; Matsumoto, S; Teague, J C and Krohn, K A (2002) In Diabetes Technology & Therapeutics 4(1). p.67-76
Abstract
Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells... (More)
Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes Technology & Therapeutics
volume
4
issue
1
pages
67 - 76
publisher
Mary Ann Liebert, Inc.
external identifiers
  • scopus:0036232564
ISSN
1520-9156
DOI
10.1089/15209150252924111
language
English
LU publication?
no
id
dc293cca-bec1-4e60-9a94-3c56d82b7e3a (old id 1124810)
date added to LUP
2008-06-03 11:23:00
date last changed
2017-01-29 03:37:17
@article{dc293cca-bec1-4e60-9a94-3c56d82b7e3a,
  abstract     = {Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a},
  author       = {Sweet, I R and Cook, D L and Wiseman, R W and Greenbaum, C J and Lernmark, Åke and Matsumoto, S and Teague, J C and Krohn, K A},
  issn         = {1520-9156},
  language     = {eng},
  number       = {1},
  pages        = {67--76},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Diabetes Technology & Therapeutics},
  title        = {Dynamic perifusion to maintain and assess isolated pancreatic islets.},
  url          = {http://dx.doi.org/10.1089/15209150252924111},
  volume       = {4},
  year         = {2002},
}