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Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit

Saleem, A; Michael, H; Härkönen, Pirkko LU and Pihlaja, K (2002) In Journal of Ethnopharmacology 81(3). p.327-336
Abstract
A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the... (More)
A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the extract at lower concentrations, but at higher concentrations, necrosis was the major mechanism of cell death. ATP assay guided chromatographic fractionation of the extract yielded ellagic acid, 2,4-chebulyl-β-Image -glucopyranose (a new natural product), and chebulinic acid which were tested by ATP assay on HOS-1 cell line in comparison to three known antigrowth phenolics of Terminalia, gallic acid, ethyl gallate, luteolin, and tannic acid. Chebulinic acid (IC50=53.2 μM±0.16)>tannic acid (IC50=59.0 μg/ml±0.19)> and ellagic acid (IC50=78.5 μM±0.24), were the most growth inhibitory phenolics of T. chebula fruit in our study. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hydrolyzable tannins, Phenolics, Apoptosis, Cytotoxicity, Terminalia chebula Retz.
in
Journal of Ethnopharmacology
volume
81
issue
3
pages
327 - 336
publisher
Elsevier
external identifiers
  • scopus:0035996866
ISSN
1872-7573
DOI
10.1016/S0378-8741(02)00099-5
language
English
LU publication?
no
id
32b73865-ea1c-4852-a0ec-30e5accd04cf (old id 1124937)
date added to LUP
2008-05-30 08:09:48
date last changed
2017-12-10 03:47:56
@article{32b73865-ea1c-4852-a0ec-30e5accd04cf,
  abstract     = {A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the extract at lower concentrations, but at higher concentrations, necrosis was the major mechanism of cell death. ATP assay guided chromatographic fractionation of the extract yielded ellagic acid, 2,4-chebulyl-β-Image -glucopyranose (a new natural product), and chebulinic acid which were tested by ATP assay on HOS-1 cell line in comparison to three known antigrowth phenolics of Terminalia, gallic acid, ethyl gallate, luteolin, and tannic acid. Chebulinic acid (IC50=53.2 μM±0.16)>tannic acid (IC50=59.0 μg/ml±0.19)> and ellagic acid (IC50=78.5 μM±0.24), were the most growth inhibitory phenolics of T. chebula fruit in our study.},
  author       = {Saleem, A and Michael, H and Härkönen, Pirkko and Pihlaja, K},
  issn         = {1872-7573},
  keyword      = {Hydrolyzable tannins,Phenolics,Apoptosis,Cytotoxicity,Terminalia chebula Retz.},
  language     = {eng},
  number       = {3},
  pages        = {327--336},
  publisher    = {Elsevier},
  series       = {Journal of Ethnopharmacology},
  title        = {Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit},
  url          = {http://dx.doi.org/10.1016/S0378-8741(02)00099-5},
  volume       = {81},
  year         = {2002},
}