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Important differences in nitric oxide synthase activity and predominant isoform in reproductive tissues from human and rat.

Batra, Satish LU ; Iosif, Constantin LU ; Al-Hijji, J and Larsson, Irene LU (2003) In Reproductive Biology and Endocrinology p.10-10
Abstract
For the extrapolation of data obtained from experimental animals to the human situation, it is important to know the similarities and differences between human and animal species. Some important characteristics of nitric oxide synthase (NOS) in myometrium and vagina from human and rat were compared. NOS-activity was measured by the formation of 14C-citrulline from 14C-arginine and the expression of NOS isoforms was examined by Western blotting. NOS activity in human uterus and vagina was significantly lower than in the tissues from rat. In contrast to the rat where NOS activity was predominantly found in the cytosolic fractions, NOS activity in particulate and cytosolic fractions from both human myometrium and vagina was similar. Data from... (More)
For the extrapolation of data obtained from experimental animals to the human situation, it is important to know the similarities and differences between human and animal species. Some important characteristics of nitric oxide synthase (NOS) in myometrium and vagina from human and rat were compared. NOS-activity was measured by the formation of 14C-citrulline from 14C-arginine and the expression of NOS isoforms was examined by Western blotting. NOS activity in human uterus and vagina was significantly lower than in the tissues from rat. In contrast to the rat where NOS activity was predominantly found in the cytosolic fractions, NOS activity in particulate and cytosolic fractions from both human myometrium and vagina was similar. Data from Western blots confirmed that eNOS and nNOS isoforms were concentrated in the particulate and cytosolic fractions, respectively. Estrogen treatment of rats resulted in a down regulation of uterine cytosolic NOS activity. A down regulation of NOS in the cytosolic fraction was also seen in the human pregnant myometrium as compared with the nonpregnant myometrium. The vaginal NOS activity was considerably higher than the uterus in both species. In spite of some clear-cut qualitative and other differences between human and rat tissues, there are some interesting similarities. Downregulation in pregnancy of human uterine NOS is probably due to, at least in part, the influence of estrogen and progesterone. (Less)
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organization
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Contribution to journal
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published
subject
in
Reproductive Biology and Endocrinology
pages
10 - 10
publisher
BioMed Central
external identifiers
  • scopus:4344574938
ISSN
1477-7827
DOI
10.1186/1477-7827-1-10
language
English
LU publication?
yes
id
0f571714-f0fa-4477-9fc1-13029f70c256 (old id 112518)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=12657160&dopt=Abstract
date added to LUP
2007-07-17 16:13:03
date last changed
2018-01-07 08:44:29
@article{0f571714-f0fa-4477-9fc1-13029f70c256,
  abstract     = {For the extrapolation of data obtained from experimental animals to the human situation, it is important to know the similarities and differences between human and animal species. Some important characteristics of nitric oxide synthase (NOS) in myometrium and vagina from human and rat were compared. NOS-activity was measured by the formation of 14C-citrulline from 14C-arginine and the expression of NOS isoforms was examined by Western blotting. NOS activity in human uterus and vagina was significantly lower than in the tissues from rat. In contrast to the rat where NOS activity was predominantly found in the cytosolic fractions, NOS activity in particulate and cytosolic fractions from both human myometrium and vagina was similar. Data from Western blots confirmed that eNOS and nNOS isoforms were concentrated in the particulate and cytosolic fractions, respectively. Estrogen treatment of rats resulted in a down regulation of uterine cytosolic NOS activity. A down regulation of NOS in the cytosolic fraction was also seen in the human pregnant myometrium as compared with the nonpregnant myometrium. The vaginal NOS activity was considerably higher than the uterus in both species. In spite of some clear-cut qualitative and other differences between human and rat tissues, there are some interesting similarities. Downregulation in pregnancy of human uterine NOS is probably due to, at least in part, the influence of estrogen and progesterone.},
  author       = {Batra, Satish and Iosif, Constantin and Al-Hijji, J and Larsson, Irene},
  issn         = {1477-7827},
  language     = {eng},
  pages        = {10--10},
  publisher    = {BioMed Central},
  series       = {Reproductive Biology and Endocrinology},
  title        = {Important differences in nitric oxide synthase activity and predominant isoform in reproductive tissues from human and rat.},
  url          = {http://dx.doi.org/10.1186/1477-7827-1-10},
  year         = {2003},
}