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Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics

Albiger, Barbara LU ; Johansson, Linda and Jonsson, Ann-Beth (2003) In Infection and Immunity 71(1). p.155-155
Abstract
Molecular interaction between host mucosal surfaces and outer membrane components of microbes is crucial in the infection process. The outer membrane of pathogenic Neisseria contains surface molecules such as pili, PilC, and Opa and a monolayer of lipooligosaccharide (LOS), all of which are involved in the interaction with host cells. Pili mediate the initial attachment to human epithelial cells, which is followed by tight contact between bacteria and the eucaryotic cells, leading to bacterial invasion. To further examine the basis for bacterium-host cell contact, we constructed an LOS-deficient Neisseria meningitidis serogroup C mutant. LOS deficiency was without exception accompanied by altered colony opacity and morphology, which most... (More)
Molecular interaction between host mucosal surfaces and outer membrane components of microbes is crucial in the infection process. The outer membrane of pathogenic Neisseria contains surface molecules such as pili, PilC, and Opa and a monolayer of lipooligosaccharide (LOS), all of which are involved in the interaction with host cells. Pili mediate the initial attachment to human epithelial cells, which is followed by tight contact between bacteria and the eucaryotic cells, leading to bacterial invasion. To further examine the basis for bacterium-host cell contact, we constructed an LOS-deficient Neisseria meningitidis serogroup C mutant. LOS deficiency was without exception accompanied by altered colony opacity and morphology, which most likely represented an "on" switch for Opa540 expression, and by reduced levels of the iron-regulated proteins FetA and FbpA. We show here that LOS is essential for pilus-associated adherence but dispensable for fiber formation and twitching motility. The absence of attachment to epithelial cells could not be attributed to altered levels of piliation or defects in the pilus adhesion phenotype. Further, LOS mutants do not invade host cells and have lost the natural competence for genetic transformation. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
71
issue
1
pages
155 - 155
publisher
American Society for Microbiology
external identifiers
  • pmid:12496161
  • scopus:0037222332
ISSN
1098-5522
DOI
10.1128/IAI.71.1.155-162.2003
language
English
LU publication?
no
id
7f4a98a2-2936-498e-be57-0a52416538e8 (old id 1126306)
date added to LUP
2008-06-02 10:37:36
date last changed
2018-05-29 10:29:03
@article{7f4a98a2-2936-498e-be57-0a52416538e8,
  abstract     = {Molecular interaction between host mucosal surfaces and outer membrane components of microbes is crucial in the infection process. The outer membrane of pathogenic Neisseria contains surface molecules such as pili, PilC, and Opa and a monolayer of lipooligosaccharide (LOS), all of which are involved in the interaction with host cells. Pili mediate the initial attachment to human epithelial cells, which is followed by tight contact between bacteria and the eucaryotic cells, leading to bacterial invasion. To further examine the basis for bacterium-host cell contact, we constructed an LOS-deficient Neisseria meningitidis serogroup C mutant. LOS deficiency was without exception accompanied by altered colony opacity and morphology, which most likely represented an "on" switch for Opa540 expression, and by reduced levels of the iron-regulated proteins FetA and FbpA. We show here that LOS is essential for pilus-associated adherence but dispensable for fiber formation and twitching motility. The absence of attachment to epithelial cells could not be attributed to altered levels of piliation or defects in the pilus adhesion phenotype. Further, LOS mutants do not invade host cells and have lost the natural competence for genetic transformation.},
  author       = {Albiger, Barbara and Johansson, Linda and Jonsson, Ann-Beth},
  issn         = {1098-5522},
  language     = {eng},
  number       = {1},
  pages        = {155--155},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics},
  url          = {http://dx.doi.org/10.1128/IAI.71.1.155-162.2003},
  volume       = {71},
  year         = {2003},
}