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Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease

Kees, Ursula R; Ford, Jette; Watson, Marcia; Murch, Ashleigh; Ringnér, Markus LU ; Walker, Robert L and Meltzer, Paul (2003) In Molecular Cancer Therapeutics 2(7). p.671-677
Abstract
The development of new drugs against cancer requires established cell lines. They are needed for in vitro studies to identify candidate drugs and in xenograft models to measure drug efficacy in vivo. Specific criteria need to be fulfilled by cell lines used in the evaluation of potential novel therapeutic agents. It is imperative that they display the features of the particular cancer under investigation. Given the documented heterogeneity of cancers, relevant subtypes need to be represented. In this study, we have examined these aspects for pediatric acute lymphoblastic leukemia. A panel of 13 leukemia cell lines recently established in our laboratory was analyzed. We used cDNA microarrays to define the gene expression profiles and... (More)
The development of new drugs against cancer requires established cell lines. They are needed for in vitro studies to identify candidate drugs and in xenograft models to measure drug efficacy in vivo. Specific criteria need to be fulfilled by cell lines used in the evaluation of potential novel therapeutic agents. It is imperative that they display the features of the particular cancer under investigation. Given the documented heterogeneity of cancers, relevant subtypes need to be represented. In this study, we have examined these aspects for pediatric acute lymphoblastic leukemia. A panel of 13 leukemia cell lines recently established in our laboratory was analyzed. We used cDNA microarrays to define the gene expression profiles and compared the data with immunophenotyping and cytogenetic analyses. The expression profiles obtained showed excellent concordance with corresponding protein levels. Importantly, the panel of lines displayed the critical genetic features identified in clinically important acute lymphoblastic leukemia subtypes in childhood leukemia patients. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Cancer Therapeutics
volume
2
issue
7
pages
671 - 677
publisher
American Association for Cancer Research
external identifiers
  • pmid:12883040
  • scopus:2142686587
ISSN
1538-8514
language
English
LU publication?
no
id
13d77efe-7ff8-4e2b-9fdb-84d6948cfa31 (old id 1126433)
alternative location
http://mct.aacrjournals.org/content/2/7/671.long
date added to LUP
2008-06-02 15:31:01
date last changed
2018-01-07 06:03:59
@article{13d77efe-7ff8-4e2b-9fdb-84d6948cfa31,
  abstract     = {The development of new drugs against cancer requires established cell lines. They are needed for in vitro studies to identify candidate drugs and in xenograft models to measure drug efficacy in vivo. Specific criteria need to be fulfilled by cell lines used in the evaluation of potential novel therapeutic agents. It is imperative that they display the features of the particular cancer under investigation. Given the documented heterogeneity of cancers, relevant subtypes need to be represented. In this study, we have examined these aspects for pediatric acute lymphoblastic leukemia. A panel of 13 leukemia cell lines recently established in our laboratory was analyzed. We used cDNA microarrays to define the gene expression profiles and compared the data with immunophenotyping and cytogenetic analyses. The expression profiles obtained showed excellent concordance with corresponding protein levels. Importantly, the panel of lines displayed the critical genetic features identified in clinically important acute lymphoblastic leukemia subtypes in childhood leukemia patients.},
  author       = {Kees, Ursula R and Ford, Jette and Watson, Marcia and Murch, Ashleigh and Ringnér, Markus and Walker, Robert L and Meltzer, Paul},
  issn         = {1538-8514},
  language     = {eng},
  number       = {7},
  pages        = {671--677},
  publisher    = {American Association for Cancer Research},
  series       = {Molecular Cancer Therapeutics},
  title        = {Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease},
  volume       = {2},
  year         = {2003},
}