Sorting of soluble TNF-receptor for granule storage in hematopoietic cells as a principle for targeting of selected proteins to inflamed sites.
(2003) In Blood 102(2). p.682-688- Abstract
- Hematopoietic cells have secretory lysosomes that degranulate at the inflammatory site upon stimulation. We asked whether one could target exogenous proteins with a therapeutic potential to secretory lysosomes in hematopoietic cells. For this purpose, we expressed a soluble tumor necrosis factor (TNF) receptor form (sTNFR1) in hematopoietic cell lines. In order to accomplish targeting to secretory lysosomes, both endoplasmic reticulum (ER) retention and constitutive secretion have to be prevented. ER export was facilitated by addition of a transmembrane (tm) sequence, and constitutive secretion was overcome by incorporating a cytosolic sorting signal (Y) from CD63. This signal directed the resulting sTNFR1-tm-Y to secretory lysosomes.... (More)
- Hematopoietic cells have secretory lysosomes that degranulate at the inflammatory site upon stimulation. We asked whether one could target exogenous proteins with a therapeutic potential to secretory lysosomes in hematopoietic cells. For this purpose, we expressed a soluble tumor necrosis factor (TNF) receptor form (sTNFR1) in hematopoietic cell lines. In order to accomplish targeting to secretory lysosomes, both endoplasmic reticulum (ER) retention and constitutive secretion have to be prevented. ER export was facilitated by addition of a transmembrane (tm) sequence, and constitutive secretion was overcome by incorporating a cytosolic sorting signal (Y) from CD63. This signal directed the resulting sTNFR1-tm-Y to secretory lysosomes. Confirmation of these results was provided by biosynthetic radiolabeling, subcellular fractionation, immunofluorescence microscopy, and immunoelectron microscopy. The tm-Y fragment was cleaved by proteolysis, resulting in generation of the membrane-free sTNFR1 in secretory lysosomes. Our results suggest a potential for using the storage organelles of hematopoietic cells as vehicles for targeting sites of inflammation with therapeutically active agents. (C) 2003 by The American Society of Hematology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/112646
- author
- Gao, Ying LU ; Rosén, Hanna LU ; Johnsson, Ellinor ; Calafat, Jero ; Tapper, Hans LU and Olsson, Inge LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 102
- issue
- 2
- pages
- 682 - 688
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000184083500048
- scopus:0038156161
- pmid:12649164
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2002-10-3055
- language
- English
- LU publication?
- yes
- id
- fe15660a-0d7f-494c-bfc3-c563ed15d5b9 (old id 112646)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12649164&dopt=Abstract
- date added to LUP
- 2016-04-01 12:07:38
- date last changed
- 2022-01-26 23:08:38
@article{fe15660a-0d7f-494c-bfc3-c563ed15d5b9, abstract = {{Hematopoietic cells have secretory lysosomes that degranulate at the inflammatory site upon stimulation. We asked whether one could target exogenous proteins with a therapeutic potential to secretory lysosomes in hematopoietic cells. For this purpose, we expressed a soluble tumor necrosis factor (TNF) receptor form (sTNFR1) in hematopoietic cell lines. In order to accomplish targeting to secretory lysosomes, both endoplasmic reticulum (ER) retention and constitutive secretion have to be prevented. ER export was facilitated by addition of a transmembrane (tm) sequence, and constitutive secretion was overcome by incorporating a cytosolic sorting signal (Y) from CD63. This signal directed the resulting sTNFR1-tm-Y to secretory lysosomes. Confirmation of these results was provided by biosynthetic radiolabeling, subcellular fractionation, immunofluorescence microscopy, and immunoelectron microscopy. The tm-Y fragment was cleaved by proteolysis, resulting in generation of the membrane-free sTNFR1 in secretory lysosomes. Our results suggest a potential for using the storage organelles of hematopoietic cells as vehicles for targeting sites of inflammation with therapeutically active agents. (C) 2003 by The American Society of Hematology.}}, author = {{Gao, Ying and Rosén, Hanna and Johnsson, Ellinor and Calafat, Jero and Tapper, Hans and Olsson, Inge}}, issn = {{1528-0020}}, language = {{eng}}, number = {{2}}, pages = {{682--688}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{Sorting of soluble TNF-receptor for granule storage in hematopoietic cells as a principle for targeting of selected proteins to inflamed sites.}}, url = {{http://dx.doi.org/10.1182/blood-2002-10-3055}}, doi = {{10.1182/blood-2002-10-3055}}, volume = {{102}}, year = {{2003}}, }