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In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma

Okroj, Marcin LU ; Dobrzanska-Paprocka, Zuzanna; Rolka, Krzysztof and Bigda, Jacek (2003) In Cellular & Molecular Biology Letters 8(4). p.873-884
Abstract
The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including caspases. Synthetic RGD peptides may affect adhesion, migration and tumour metastasis, or directly induce apoptosis. Several RGD peptides were synthesised, and their anti-adhesive and cytotoxic properties were analysed in vitro. The most active peptide (poly RGD) was also tested in vivo to assess its modulatory activity on melanoma growth. Synthetic RGD peptides inhibit the adhesion of Ab melanoma cells to fibronectin. Poly RGD significantly inhibits primary tumour growth. There was no observed cytotoxicity of poly RGD towards Ab cells in a medium with 10% serum; however, under the same conditions, the anti-adhesive effect of poly RGD... (More)
The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including caspases. Synthetic RGD peptides may affect adhesion, migration and tumour metastasis, or directly induce apoptosis. Several RGD peptides were synthesised, and their anti-adhesive and cytotoxic properties were analysed in vitro. The most active peptide (poly RGD) was also tested in vivo to assess its modulatory activity on melanoma growth. Synthetic RGD peptides inhibit the adhesion of Ab melanoma cells to fibronectin. Poly RGD significantly inhibits primary tumour growth. There was no observed cytotoxicity of poly RGD towards Ab cells in a medium with 10% serum; however, under the same conditions, the anti-adhesive effect of poly RGD was still visible. Experiments on Jurkat cells indicated a weak cytotoxicity of poly RGD and a significant cytotoxicity of GRGDNP (the reference cytotoxic peptide), retained only under serum-free conditions. The anti-tumour effect of poly RGD observed in the Ab Bomirski melanoma model is probably due to an anti-adhesive mechanism. The proapoptotic activity of RGD peptides is dependent on the absence of serum. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Melanoma, Tumour, Adhesion, RGD
in
Cellular & Molecular Biology Letters
volume
8
issue
4
pages
873 - 884
publisher
Versita
external identifiers
  • pmid:14668910
  • scopus:0347087422
ISSN
1689-1392
language
English
LU publication?
yes
id
42af3024-2053-4d85-8468-9dad9922d16f (old id 1126461)
date added to LUP
2008-06-11 11:46:58
date last changed
2018-05-29 11:52:18
@article{42af3024-2053-4d85-8468-9dad9922d16f,
  abstract     = {The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including caspases. Synthetic RGD peptides may affect adhesion, migration and tumour metastasis, or directly induce apoptosis. Several RGD peptides were synthesised, and their anti-adhesive and cytotoxic properties were analysed in vitro. The most active peptide (poly RGD) was also tested in vivo to assess its modulatory activity on melanoma growth. Synthetic RGD peptides inhibit the adhesion of Ab melanoma cells to fibronectin. Poly RGD significantly inhibits primary tumour growth. There was no observed cytotoxicity of poly RGD towards Ab cells in a medium with 10% serum; however, under the same conditions, the anti-adhesive effect of poly RGD was still visible. Experiments on Jurkat cells indicated a weak cytotoxicity of poly RGD and a significant cytotoxicity of GRGDNP (the reference cytotoxic peptide), retained only under serum-free conditions. The anti-tumour effect of poly RGD observed in the Ab Bomirski melanoma model is probably due to an anti-adhesive mechanism. The proapoptotic activity of RGD peptides is dependent on the absence of serum.},
  author       = {Okroj, Marcin and Dobrzanska-Paprocka, Zuzanna and Rolka, Krzysztof and Bigda, Jacek},
  issn         = {1689-1392},
  keyword      = {Melanoma,Tumour,Adhesion,RGD},
  language     = {eng},
  number       = {4},
  pages        = {873--884},
  publisher    = {Versita},
  series       = {Cellular & Molecular Biology Letters},
  title        = {In vitro and in vivo analyses of the biological activity of RGD peptides towards Ab Bomirski melanoma},
  volume       = {8},
  year         = {2003},
}