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DNA analysis for donor screening of Dombrock blood group antigens

Storry, Jill LU ; Westhoff, C M; Charles-Pierre, D; Rios, M; Hue-Roye, K; Vege, S; Nance, S and Reid, M E (2003) In Immunohematology 19(3). p.73-76
Abstract
Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes:378 C>T, 624 T>C,and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob. We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected... (More)
Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes:378 C>T, 624 T>C,and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob. We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCRRFLP. PCR-RFLP can be used to screen for Do(a-) or Do(b-) donors. This approach overcomes the scarcity of the reagents required for testing by hemagglutination. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR-RFLP, Dombrock blood group system, Dombrock polymorphisms Doa/Dob, typing donors
in
Immunohematology
volume
19
issue
3
pages
73 - 76
publisher
American Red Cross
external identifiers
  • pmid:15373684
  • scopus:0041384471
ISSN
0894-203X
language
English
LU publication?
no
id
7de404c6-20d0-4f4f-89e4-3d392c719514 (old id 1126484)
alternative location
http://www.redcross.org/pubs/immuno/19_3sm.pdf
date added to LUP
2008-06-11 10:27:11
date last changed
2018-01-07 09:17:18
@article{7de404c6-20d0-4f4f-89e4-3d392c719514,
  abstract     = {Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes:378 C>T, 624 T>C,and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob. We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCRRFLP. PCR-RFLP can be used to screen for Do(a-) or Do(b-) donors. This approach overcomes the scarcity of the reagents required for testing by hemagglutination.},
  author       = {Storry, Jill and Westhoff, C M and Charles-Pierre, D and Rios, M and Hue-Roye, K and Vege, S and Nance, S and Reid, M E},
  issn         = {0894-203X},
  keyword      = {PCR-RFLP,Dombrock blood group system,Dombrock polymorphisms Doa/Dob,typing
donors},
  language     = {eng},
  number       = {3},
  pages        = {73--76},
  publisher    = {American Red Cross},
  series       = {Immunohematology},
  title        = {DNA analysis for donor screening of Dombrock blood group antigens},
  volume       = {19},
  year         = {2003},
}