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GUTI: a new antigen in the Cromer blood group system

Storry, Jill LU ; Sausais, Laima; Hue-Roye, Kim; Mudiwa, Flora; Ferrer, Zennie; Blajchman, Morris A; Lublin, Douglas M; Ma, Bei-Wen; Miquel, Juan F and Nervi, Flavio, et al. (2003) In Transfusion 43(3). p.340-344
Abstract
BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for... (More)
BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4. CONCLUSION: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position. (Less)
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type
Contribution to journal
publication status
published
subject
in
Transfusion
volume
43
issue
3
pages
340 - 344
publisher
Wiley-Blackwell
external identifiers
  • pmid:12675719
  • scopus:0037342364
ISSN
1537-2995
DOI
10.1046/j.1537-2995.2003.00319.x
language
English
LU publication?
no
id
bc4cff75-b87b-4caa-9af4-73460e753a4e (old id 1126512)
date added to LUP
2008-06-11 10:42:14
date last changed
2017-01-01 07:24:16
@article{bc4cff75-b87b-4caa-9af4-73460e753a4e,
  abstract     = {BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4. CONCLUSION: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.},
  author       = {Storry, Jill and Sausais, Laima and Hue-Roye, Kim and Mudiwa, Flora and Ferrer, Zennie and Blajchman, Morris A and Lublin, Douglas M and Ma, Bei-Wen and Miquel, Juan F and Nervi, Flavio and Pereira, Jaime and Reid, Marion E},
  issn         = {1537-2995},
  language     = {eng},
  number       = {3},
  pages        = {340--344},
  publisher    = {Wiley-Blackwell},
  series       = {Transfusion},
  title        = {GUTI: a new antigen in the Cromer blood group system},
  url          = {http://dx.doi.org/10.1046/j.1537-2995.2003.00319.x},
  volume       = {43},
  year         = {2003},
}