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Dysregulated secretoglobin expression in human lung cancers

Sjodin, Anna; Guo, Dongsheng; Sorhaug, Sveinung; Bjermer, Leif LU ; Henriksson, Roger and Hedman, Hakan (2003) In Lung Cancer 41(1). p.49-56
Abstract
Lipophilins A, B, C, mammaglobin, and uteroglobin are members of the secretoglobin family of small, secreted, proteins. The functions of these proteins are not well understood but uteroglobin has been implicated in the development of cancers. Uteroglobin is known to be highly expressed in normal lung and down-regulated in lung cancers but expression of the other secretoglobins in normal lung and lung neoplasms have not been investigated. Therefore, we developed quantitative real-time reverse transcription (RT-) PCR assays for the different secretoglobins and evaluated their expression in normal and neoplastic lung tissues. The secretoglobin transcript levels were quantitated by real-time RT-PCR in samples from three normal lungs, 24 lung... (More)
Lipophilins A, B, C, mammaglobin, and uteroglobin are members of the secretoglobin family of small, secreted, proteins. The functions of these proteins are not well understood but uteroglobin has been implicated in the development of cancers. Uteroglobin is known to be highly expressed in normal lung and down-regulated in lung cancers but expression of the other secretoglobins in normal lung and lung neoplasms have not been investigated. Therefore, we developed quantitative real-time reverse transcription (RT-) PCR assays for the different secretoglobins and evaluated their expression in normal and neoplastic lung tissues. The secretoglobin transcript levels were quantitated by real-time RT-PCR in samples from three normal lungs, 24 lung tumors including six small cell lung carcinomas, seven adenocarcinomas, and five squamous cell carcinomas, and in cell lines from three small cell lung carcinomas and one mesothelioma. Uteroglobin was confirmed to be abundantly expressed in normal lung and the different lung tumors showed down-regulated uteroglobin expression. Of the other secretoglobins, only lipophilin C was detected in normal lung, albeit at low levels. The lung tumors, however, frequently showed neo- or up-regulation of lipophilins A, B, C, and mammaglobin. The results constitute the first quantitative evaluation of secretoglobin expression in normal and neoplastic human lung tissues and demonstrate dysregulation in various human lung cancers. These findings could have important biological and diagnostic implications. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Lung Cancer
volume
41
issue
1
pages
49 - 56
publisher
Elsevier
external identifiers
  • pmid:12826312
  • scopus:0038121537
ISSN
1872-8332
DOI
10.1016/S0169-5002(03)00126-0
language
English
LU publication?
no
id
f4757461-f1f8-4633-96f2-4e904242658b (old id 1126565)
date added to LUP
2008-06-11 11:16:32
date last changed
2018-01-07 06:19:08
@article{f4757461-f1f8-4633-96f2-4e904242658b,
  abstract     = {Lipophilins A, B, C, mammaglobin, and uteroglobin are members of the secretoglobin family of small, secreted, proteins. The functions of these proteins are not well understood but uteroglobin has been implicated in the development of cancers. Uteroglobin is known to be highly expressed in normal lung and down-regulated in lung cancers but expression of the other secretoglobins in normal lung and lung neoplasms have not been investigated. Therefore, we developed quantitative real-time reverse transcription (RT-) PCR assays for the different secretoglobins and evaluated their expression in normal and neoplastic lung tissues. The secretoglobin transcript levels were quantitated by real-time RT-PCR in samples from three normal lungs, 24 lung tumors including six small cell lung carcinomas, seven adenocarcinomas, and five squamous cell carcinomas, and in cell lines from three small cell lung carcinomas and one mesothelioma. Uteroglobin was confirmed to be abundantly expressed in normal lung and the different lung tumors showed down-regulated uteroglobin expression. Of the other secretoglobins, only lipophilin C was detected in normal lung, albeit at low levels. The lung tumors, however, frequently showed neo- or up-regulation of lipophilins A, B, C, and mammaglobin. The results constitute the first quantitative evaluation of secretoglobin expression in normal and neoplastic human lung tissues and demonstrate dysregulation in various human lung cancers. These findings could have important biological and diagnostic implications.},
  author       = {Sjodin, Anna and Guo, Dongsheng and Sorhaug, Sveinung and Bjermer, Leif and Henriksson, Roger and Hedman, Hakan},
  issn         = {1872-8332},
  language     = {eng},
  number       = {1},
  pages        = {49--56},
  publisher    = {Elsevier},
  series       = {Lung Cancer},
  title        = {Dysregulated secretoglobin expression in human lung cancers},
  url          = {http://dx.doi.org/10.1016/S0169-5002(03)00126-0},
  volume       = {41},
  year         = {2003},
}