Apamin inhibits NO-induced relaxation of the spontaneous contractile activity of the myometrium from non-pregnant women
(2003) In Reproductive Biology and Endocrinology 1(8).- Abstract
- There is now considerable evidence for the involvement of K+ channels in nitric oxide (NO) induced relaxation of smooth muscles including the myometrium. In order to assess whether apamin-sensitive K+ channels play a role in NO - induced relaxation of the human uterus, we have studied the effect of specific blockers of these channels on the relaxation of myometrium from non-pregnant women. In vitro isometric contractions were recorded in uterine tissues from non-pregnant premenopausal women who had undergone hysterectomy. Apamin (10 nM) and scyllatoxin (10 nM) did not alter spontaneous myometrial contractions. However, 15-min pretreatment of the myometrium strips with apamin completely inhibited relaxation caused by diethylamine-nitric... (More)
- There is now considerable evidence for the involvement of K+ channels in nitric oxide (NO) induced relaxation of smooth muscles including the myometrium. In order to assess whether apamin-sensitive K+ channels play a role in NO - induced relaxation of the human uterus, we have studied the effect of specific blockers of these channels on the relaxation of myometrium from non-pregnant women. In vitro isometric contractions were recorded in uterine tissues from non-pregnant premenopausal women who had undergone hysterectomy. Apamin (10 nM) and scyllatoxin (10 nM) did not alter spontaneous myometrial contractions. However, 15-min pretreatment of the myometrium strips with apamin completely inhibited relaxation caused by diethylamine-nitric oxide (DEA/NO). The pretreatment with scyllatoxin significantly reduced (about 2.6 times) maximum relaxation of the strips induced by DEA/NO (p < 0.05). These results strongly suggest that, beside Ca2+ and voltage dependent charybdotoxin-sensitive (CTX-sensitive) K+ channels, apamin-sensitive K+ channels are also present in the human non-pregnant myometrium. These channels offer an additional target in the development of new tocolytic agents. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1126825
- author
- Modzelewska, Beata ; Kostrzewska, Anna ; Sipowicz, Marek ; Kleszczewski, Tomasz and Batra, Satish LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Reproductive Biology and Endocrinology
- volume
- 1
- issue
- 8
- article number
- 8
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:12646073
- scopus:4344605038
- ISSN
- 1477-7827
- DOI
- 10.1186/1477-7827-1-8
- language
- English
- LU publication?
- yes
- id
- 76ece3a7-229d-4290-879b-0b9025d45b3e (old id 1126825)
- date added to LUP
- 2016-04-01 16:34:14
- date last changed
- 2022-01-28 20:34:08
@article{76ece3a7-229d-4290-879b-0b9025d45b3e, abstract = {{There is now considerable evidence for the involvement of K+ channels in nitric oxide (NO) induced relaxation of smooth muscles including the myometrium. In order to assess whether apamin-sensitive K+ channels play a role in NO - induced relaxation of the human uterus, we have studied the effect of specific blockers of these channels on the relaxation of myometrium from non-pregnant women. In vitro isometric contractions were recorded in uterine tissues from non-pregnant premenopausal women who had undergone hysterectomy. Apamin (10 nM) and scyllatoxin (10 nM) did not alter spontaneous myometrial contractions. However, 15-min pretreatment of the myometrium strips with apamin completely inhibited relaxation caused by diethylamine-nitric oxide (DEA/NO). The pretreatment with scyllatoxin significantly reduced (about 2.6 times) maximum relaxation of the strips induced by DEA/NO (p < 0.05). These results strongly suggest that, beside Ca2+ and voltage dependent charybdotoxin-sensitive (CTX-sensitive) K+ channels, apamin-sensitive K+ channels are also present in the human non-pregnant myometrium. These channels offer an additional target in the development of new tocolytic agents.}}, author = {{Modzelewska, Beata and Kostrzewska, Anna and Sipowicz, Marek and Kleszczewski, Tomasz and Batra, Satish}}, issn = {{1477-7827}}, language = {{eng}}, number = {{8}}, publisher = {{BioMed Central (BMC)}}, series = {{Reproductive Biology and Endocrinology}}, title = {{Apamin inhibits NO-induced relaxation of the spontaneous contractile activity of the myometrium from non-pregnant women}}, url = {{http://dx.doi.org/10.1186/1477-7827-1-8}}, doi = {{10.1186/1477-7827-1-8}}, volume = {{1}}, year = {{2003}}, }