The RING-H2 protein RNF11 is differentially expressed in breast tumours and interacts with HECT-type E3 ligases
(2003) In Biochimica et Biophysica Acta 1639(2). p.104-112- Abstract
- A breast cancer-associated mRNA originally cloned as a 475-bp partial cDNA from a library enriched for tumour cDNAs [Oncogene 16 (1998) 327] is expressed at high levels in breast and prostate cancer cells. Immunohistochemical analysis indicates that the protein is expressed in primary breast tumours. We used RT-PCR to generate a full-length 2852 nt mRNA sequence that includes the hypothetical open reading frame (ORF) for human RNF11. Our analysis shows that RNF11 encodes modular domains and motifs likely to interact with other proteins involved in oncogenesis. Chief among these are the RING-H2 finger domain that could facilitate the degradation of specific substrate(s) involved in oncogenesis and the PY motif which binds to WW-domain... (More)
- A breast cancer-associated mRNA originally cloned as a 475-bp partial cDNA from a library enriched for tumour cDNAs [Oncogene 16 (1998) 327] is expressed at high levels in breast and prostate cancer cells. Immunohistochemical analysis indicates that the protein is expressed in primary breast tumours. We used RT-PCR to generate a full-length 2852 nt mRNA sequence that includes the hypothetical open reading frame (ORF) for human RNF11. Our analysis shows that RNF11 encodes modular domains and motifs likely to interact with other proteins involved in oncogenesis. Chief among these are the RING-H2 finger domain that could facilitate the degradation of specific substrate(s) involved in oncogenesis and the PY motif which binds to WW-domain proteins, several of which are known to be E3 ubiquitin ligases. Our GST-pulldown and immunoprecipitation results indicate that RNF11 interacts with the E3 ligase AIP4 when coexpressed with RNF11 in mammalian cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127179
- author
- Kitching, Richard ; Wong, Michael J ; Koehler, David ; Burger, Angelika M ; Landberg, Göran LU ; Gish, Gerald and Seth, Arun
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta
- volume
- 1639
- issue
- 2
- pages
- 104 - 112
- publisher
- Elsevier
- external identifiers
-
- pmid:14559117
- scopus:1642414402
- ISSN
- 0006-3002
- DOI
- 10.1016/j.bbadis.2003.07.001
- language
- English
- LU publication?
- no
- id
- 1c7227db-cc54-41b8-b75e-cbb004a2594f (old id 1127179)
- date added to LUP
- 2016-04-01 16:02:09
- date last changed
- 2022-01-28 08:49:48
@article{1c7227db-cc54-41b8-b75e-cbb004a2594f, abstract = {{A breast cancer-associated mRNA originally cloned as a 475-bp partial cDNA from a library enriched for tumour cDNAs [Oncogene 16 (1998) 327] is expressed at high levels in breast and prostate cancer cells. Immunohistochemical analysis indicates that the protein is expressed in primary breast tumours. We used RT-PCR to generate a full-length 2852 nt mRNA sequence that includes the hypothetical open reading frame (ORF) for human RNF11. Our analysis shows that RNF11 encodes modular domains and motifs likely to interact with other proteins involved in oncogenesis. Chief among these are the RING-H2 finger domain that could facilitate the degradation of specific substrate(s) involved in oncogenesis and the PY motif which binds to WW-domain proteins, several of which are known to be E3 ubiquitin ligases. Our GST-pulldown and immunoprecipitation results indicate that RNF11 interacts with the E3 ligase AIP4 when coexpressed with RNF11 in mammalian cells.}}, author = {{Kitching, Richard and Wong, Michael J and Koehler, David and Burger, Angelika M and Landberg, Göran and Gish, Gerald and Seth, Arun}}, issn = {{0006-3002}}, language = {{eng}}, number = {{2}}, pages = {{104--112}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta}}, title = {{The RING-H2 protein RNF11 is differentially expressed in breast tumours and interacts with HECT-type E3 ligases}}, url = {{http://dx.doi.org/10.1016/j.bbadis.2003.07.001}}, doi = {{10.1016/j.bbadis.2003.07.001}}, volume = {{1639}}, year = {{2003}}, }