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Blood volume assessment with hemoglobin subtype analysis in preterm infants

Leipala, Jaana A; Talme, Marjo; Viitala, Juha; Turpeinen, Ursula and Fellman, Vineta LU (2003) In Biology of the Neonate 84(1). p.41-44
Abstract
The aim of the study was to assess plasma volume (PV) and red cell volume (RCV) in preterm infants with a liquid chromatographic hemoglobin subtype method (HbST) based on dilution of autologous fetal hemoglobin (HbF) by donor adult hemoglobin and measurement of the amount of HbF. PV determination with the indocyanine green dye dilution technique (ICG) was used as a reference method. Eight infants, median gestational age 29.8 weeks (range 27.6-30.9 weeks) and median birth weight 1,300 g (range 1,030-1,760 g), were studied at a median age of 3.0 days (range 1-6 days). RCV was 33.6 +/- 12 ml/kg measured with ICG and 32.1 +/- 5.2 ml/kg with HbST. PV was 47.0 +/- 18 and 40.2 +/- 6.6 ml/kg, respectively. There was a close correlation between the... (More)
The aim of the study was to assess plasma volume (PV) and red cell volume (RCV) in preterm infants with a liquid chromatographic hemoglobin subtype method (HbST) based on dilution of autologous fetal hemoglobin (HbF) by donor adult hemoglobin and measurement of the amount of HbF. PV determination with the indocyanine green dye dilution technique (ICG) was used as a reference method. Eight infants, median gestational age 29.8 weeks (range 27.6-30.9 weeks) and median birth weight 1,300 g (range 1,030-1,760 g), were studied at a median age of 3.0 days (range 1-6 days). RCV was 33.6 +/- 12 ml/kg measured with ICG and 32.1 +/- 5.2 ml/kg with HbST. PV was 47.0 +/- 18 and 40.2 +/- 6.6 ml/kg, respectively. There was a close correlation between the RCVs measured with the two techniques (Pearson correlation 0.83, p < 0.05). In conclusion, HbST provides a reliable and safe determination of RCV in preterm infants. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biology of the Neonate
volume
84
issue
1
pages
41 - 44
publisher
Karger
external identifiers
  • pmid:12890935
  • scopus:0042173121
ISSN
1421-9727
DOI
10.1159/000071442
language
English
LU publication?
no
id
1331b143-fb3e-41ae-9c7d-c46980d51774 (old id 1127349)
date added to LUP
2008-06-09 11:17:50
date last changed
2018-05-29 09:42:24
@article{1331b143-fb3e-41ae-9c7d-c46980d51774,
  abstract     = {The aim of the study was to assess plasma volume (PV) and red cell volume (RCV) in preterm infants with a liquid chromatographic hemoglobin subtype method (HbST) based on dilution of autologous fetal hemoglobin (HbF) by donor adult hemoglobin and measurement of the amount of HbF. PV determination with the indocyanine green dye dilution technique (ICG) was used as a reference method. Eight infants, median gestational age 29.8 weeks (range 27.6-30.9 weeks) and median birth weight 1,300 g (range 1,030-1,760 g), were studied at a median age of 3.0 days (range 1-6 days). RCV was 33.6 +/- 12 ml/kg measured with ICG and 32.1 +/- 5.2 ml/kg with HbST. PV was 47.0 +/- 18 and 40.2 +/- 6.6 ml/kg, respectively. There was a close correlation between the RCVs measured with the two techniques (Pearson correlation 0.83, p &lt; 0.05). In conclusion, HbST provides a reliable and safe determination of RCV in preterm infants.},
  author       = {Leipala, Jaana A and Talme, Marjo and Viitala, Juha and Turpeinen, Ursula and Fellman, Vineta},
  issn         = {1421-9727},
  language     = {eng},
  number       = {1},
  pages        = {41--44},
  publisher    = {Karger},
  series       = {Biology of the Neonate},
  title        = {Blood volume assessment with hemoglobin subtype analysis in preterm infants},
  url          = {http://dx.doi.org/10.1159/000071442},
  volume       = {84},
  year         = {2003},
}