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Unique epitopes of glutamic acid decarboxylase autoantibodies in slowly progressive type 1 diabetes.

Kobayashi, Tetsuro; Tanaka, Shoichiro; Okubo, Minoru; Nakanishi, Koji; Murase, Toshio and Lernmark, Åke LU (2003) In Journal of Clinical Endocrinology and Metabolism 88(10). p.4768-4775
Abstract
Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in... (More)
Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in SPIDDM inversely correlated with the period in which insulin was not required. GAD65Ab in AIDDM did not react with N-terminal epitope located between amino acids 1-83, irrespective of the titer of GAD65Ab. A novel epitope of GAD65Ab in AIDDM residing (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Endocrinology and Metabolism
volume
88
issue
10
pages
4768 - 4775
publisher
The Endocrine Society
external identifiers
  • scopus:0242351804
ISSN
1945-7197
DOI
10.1210/jc.2002-021529
language
English
LU publication?
no
id
3d2ac1be-60fe-45ed-8534-0674453d9cd3 (old id 1127396)
date added to LUP
2008-06-09 12:58:17
date last changed
2018-01-07 08:37:37
@article{3d2ac1be-60fe-45ed-8534-0674453d9cd3,
  abstract     = {Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in SPIDDM inversely correlated with the period in which insulin was not required. GAD65Ab in AIDDM did not react with N-terminal epitope located between amino acids 1-83, irrespective of the titer of GAD65Ab. A novel epitope of GAD65Ab in AIDDM residing},
  author       = {Kobayashi, Tetsuro and Tanaka, Shoichiro and Okubo, Minoru and Nakanishi, Koji and Murase, Toshio and Lernmark, Åke},
  issn         = {1945-7197},
  language     = {eng},
  number       = {10},
  pages        = {4768--4775},
  publisher    = {The Endocrine Society},
  series       = {Journal of Clinical Endocrinology and Metabolism},
  title        = {Unique epitopes of glutamic acid decarboxylase autoantibodies in slowly progressive type 1 diabetes.},
  url          = {http://dx.doi.org/10.1210/jc.2002-021529},
  volume       = {88},
  year         = {2003},
}