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Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.

Carroll, Andrea D; Scampavia, Louis; Luo, Dong; Lernmark, Åke LU and Ruzicka, Jaromir (2003) In Analyst 128(Sep). p.1157-1162
Abstract
This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to... (More)
This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
128
issue
Sep
pages
1157 - 1162
publisher
Royal Society of Chemistry
external identifiers
  • scopus:0141762667
ISSN
1364-5528
DOI
10.1039/b302786d
language
English
LU publication?
no
id
36acbda1-922f-4110-966b-19766bfe2c7b (old id 1127398)
date added to LUP
2008-06-09 13:08:00
date last changed
2018-05-29 11:32:16
@article{36acbda1-922f-4110-966b-19766bfe2c7b,
  abstract     = {This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection.},
  author       = {Carroll, Andrea D and Scampavia, Louis and Luo, Dong and Lernmark, Åke and Ruzicka, Jaromir},
  issn         = {1364-5528},
  language     = {eng},
  number       = {Sep},
  pages        = {1157--1162},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.},
  url          = {http://dx.doi.org/10.1039/b302786d},
  volume       = {128},
  year         = {2003},
}