Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.
(2003) In Analyst 128(Sep). p.1157-1162- Abstract
- This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to... (More)
- This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127398
- author
- Carroll, Andrea D ; Scampavia, Louis ; Luo, Dong ; Lernmark, Åke LU and Ruzicka, Jaromir
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analyst
- volume
- 128
- issue
- Sep
- pages
- 1157 - 1162
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:0141762667
- ISSN
- 1364-5528
- DOI
- 10.1039/b302786d
- language
- English
- LU publication?
- no
- id
- 36acbda1-922f-4110-966b-19766bfe2c7b (old id 1127398)
- date added to LUP
- 2016-04-01 15:54:44
- date last changed
- 2022-01-28 08:00:31
@article{36acbda1-922f-4110-966b-19766bfe2c7b, abstract = {{This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection.}}, author = {{Carroll, Andrea D and Scampavia, Louis and Luo, Dong and Lernmark, Åke and Ruzicka, Jaromir}}, issn = {{1364-5528}}, language = {{eng}}, number = {{Sep}}, pages = {{1157--1162}}, publisher = {{Royal Society of Chemistry}}, series = {{Analyst}}, title = {{Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.}}, url = {{http://dx.doi.org/10.1039/b302786d}}, doi = {{10.1039/b302786d}}, volume = {{128}}, year = {{2003}}, }