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Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay

Luo, Dong; Rogers, Chad N; Hampe, Christiane S and Lernmark, Åke LU (2003) In Journal of Biotechnology 103(3). p.249-255
Abstract
We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
103
issue
3
pages
249 - 255
publisher
Elsevier
external identifiers
  • scopus:0142059622
ISSN
1873-4863
DOI
10.1016/S0168-1656(03)00125-1
language
English
LU publication?
no
id
f635666b-9de3-47d5-9709-55f44b726f5d (old id 1127400)
date added to LUP
2008-06-09 13:14:12
date last changed
2018-01-07 05:34:57
@article{f635666b-9de3-47d5-9709-55f44b726f5d,
  abstract     = {We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system. The Biotin14-GAD65 protein exhibited the strongest binding to both the GAD65 antibody and the streptavidin among the three constructs. We describe the optimal conditions using a Biotin14-GAD65-based immunoassay for the detection of GAD65 antibody.},
  author       = {Luo, Dong and Rogers, Chad N and Hampe, Christiane S and Lernmark, Åke},
  issn         = {1873-4863},
  language     = {eng},
  number       = {3},
  pages        = {249--255},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay},
  url          = {http://dx.doi.org/10.1016/S0168-1656(03)00125-1},
  volume       = {103},
  year         = {2003},
}